- Go to File → Virtual Media Manager
- In the Hard drives tab, you should see a red exclamation point icon beside the disk image you're having trouble with. Select it → Release → Remove → Close.
- Go back into the settings for the VM and re-add the disk.
To prevent the error in the future, before deleting the virtual disk image files, remove them from the Virtual Media Manager:
- Go to File → Virtual Media Manager
- In the Hard drives tab, find the virtual disk you want to remove, select it → Release → Remove → Keep (or Delete if you want to delete it permanently) → Close.
- Go back into the settings for the VM and readd the disk.