scImpute——An accurate and robust imputation method scImpute for single-cell RNA-seq data

An accurate and robust imputation method scImpute for single-cell RNA-seq data

http://jsb.ucla.edu/sites/default/files/publications/NC_scImpute.pdf

18年UCLA刚发在NC上的一篇technology的文章，软件试过有用但是在本人数据上并没有明显的优越性，有待更多考证。

不过原理需求甚解以便效尤后续之分析。

the workflow in the imputation step of scImpute method. scImpute first learns each gene’s dropout probability in each cell by fitting a mixture model. Next, scImpute imputes the (highly probable) dropout values in cell j (gene set Aj ) by borrowing information of the same gene in other similar cells, which are selected based on gene set Bj (not severely affected by dropout events).

 selecting matrix 的图示原理：

与其他软件的比较，空白对照和平行对照：scimpute、MAGIC、SAVER 

 

one more dimension reduction plot： 

 

clustering：

效果先摆上来，确实很不错。那么具体算法和统计模型是如何架构的呢：

step by step Factorization：

 

establishing the normalized count matrix</