神舟微型计算机hfmpbvx2,Hyperthermia after nano magnetic particles embolization VX_2 rabbit liver tumor mode...

摘要：

Objective To observe the effect of hyperthermia and the influence of heat to surrounding organs of VX2 rabbit liver cancer model exposed to alternating magnetic field after embolized with nanomagnetic particles. Methods 40 rabbits with VX2 tumor were divided into four groups equally. Group A as control group receive no treatment, group B embolized with lipiodol embolization only, group C embolized with lipiodol plus magnetic particles and group D exposed to alternating magnetic field after embolized with lipiodol plus nanomagnetic particles. 14 days after the tumor transplanted, opened the abdomen to observe the size and shape of the tumor. Then trans-femoral retrograde approach of hepatic arterial catheterization was used in group B, C and D to do angiography and embolization. The next day, three models of group B and all of group D were exposed to alternating magnetic field, the temperature in the center of tumor, the edge of tumor and normal liver parenchyma were measured simultaneously. Venous blood of each animal was get just before the operation, the blood routine and the function of liver and kidney was examined. 14 days later, all animals will be killed to observe the change of tumor in the liver and the condition of the abdomen, the pathology examination will also be done. Results The volume of tumor among four groups[(1.6±1.5),(2.5±2.5),(2.7±1.6),(3.5±2.6)cm3] had no significant difference before operation(F=1.247, P=0.308). The three different positions temperature of group B under AMF had no significant difference compared to pre-operation temperature(Fcenter=0.01, Pcenter=0.981; Fedge=0.618, Pedge=0.476; Fnormol=0.217, Pnormal=0.665). Three points basic temperature of group D(35.4±1.7 ℃, 35.9±1.8 ℃ and 36.1±1.4 ℃) was without significant difference(F=1.038, P=0.413). 7 to 26 minutes after hyperthermia, the center and the edge temperature of tumor in group D had significant difference with corresponding positions of group B and liver parenchyma of group D(FB-D center=5.431, PB-D center=0.041; FB-D edge=9.744, PB-D edge=0.011; FD =8.379, PD=0.002). The highest temperature at the edge of tumor of group D could be reach 46 ℃. 14 days later, the tumor volume of 4 groups was(31.4±20.6),(26.7±18.2),(28.7±9.1),(25.8±13.9)cm3 respectively, which without significant difference with each other(F=0.218, P=0.883), but the tumor volume of Group D was the smallest. The blood routine and liver function restored to normal seven days after therapy. Conclusions It is feasible to cure the VX2 tumor under an alternating magnetic field after nanomagnetic particles embolization without significant effect on surrounding normal liver parenchyma.

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