python版本MCScanX分析方法,仅供参考:
Genomic synteny
among C. annuum, C.chinenese and C.baccatum
The genomes of C. annuum, C.chineneseandC.baccatum were retrieved from PGP ().TheC. annuumgenome was compared
toC.chineneseand C.baccatumgenomes using the MCScan toolkit (V1.1)[1].
To call synteny blocks, we performed all-against-all LAST [2] and
chained the LAST hits with a distance cutoff of 20 genes, also requiring at
least 4 gene pairs per synteny block. Chromosome-scale synteny blocks plots
were constructed using the python version of MCScan[3].
[1] Tang H, Wang X,
Bowers JE, et al. Unraveling ancient hexaploidy through multiply-aligned
angiosperm gene maps. Genome Res 2008;18(12):1944–54.
[2] S. M. Kiełbasa, R. Wan, K.
Sato, P. Horton, M. C. Frith, Adaptive seeds tame genomic sequence comparison. Genome Res. 21, 487–493 (2011). Medline
doi:10.1101/gr.113985.110
参考文献:
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