答:
如果你使用的是matlab2015b的话。
一、在GUI上添加一个坐标轴

二、把你的显示图片函数脚本放到根目录下

三、在初始化中创建计时器
timePeriod = 1.0;
t = timer('TimerFcn',{@timerCallback, handles.axes1}, 'ExecutionMode', 'fixedDelay', 'Period', timePeriod);
% 启动定时器
start(t);
四、自己写计时器函数,你的显示图片函数,在计时器函数里调用
function timerCallback(~, ~, axestemp)
mypic =LoadMyPic('图片\海绵宝宝.jpg');
axes(axestemp);
%这一句只运行一次!!就注释掉
%figure('visible','off');
imshow(mypic);
结果这样:

初次运行要运行一次 figure('visible','off'); 要不会一直有个plot的图,运行一次就注释掉。
GUI.m的全部内容
function varargout = untitled(varargin)
% UNTITLED MATLAB code for untitled.fig
% UNTITLED, by itself, creates a new UNTITLED or raises the existing
% singleton*.
%
% H = UNTITLED returns the handle to a new UNTITLED or the handle to
% the existing singleton*.
%
% UNTITLED('CALLBACK',hObject,eventData,handles,...) calls the local
% function named CALLBACK in UNTITLED.M with the given input arguments.
%
% UNTITLED('Property','Value',...) creates a new UNTITLED or raises the
% existing singleton*. Starting from the left, property value pairs are
% applied to the GUI before untitled_OpeningFcn gets called. An
% unrecognized property name or invalid value makes property application
% stop. All inputs are passed to untitled_OpeningFcn via varargin.
%
% *See GUI Options on GUIDE's Tools menu. Choose "GUI allows only one
% instance to run (singleton)".
%
% See also: GUIDE, GUIDATA, GUIHANDLES
% Edit the above text to modify the response to help untitled
% Last Modified by GUIDE v2.5 29-Mar-2023 19:27:15
% Begin initialization code - DO NOT EDIT
gui_Singleton = 1;
gui_State = struct('gui_Name', mfilename, ...
'gui_Singleton', gui_Singleton, ...
'gui_OpeningFcn', @untitled_OpeningFcn, ...
'gui_OutputFcn', @untitled_OutputFcn, ...
'gui_LayoutFcn', [] , ...
'gui_Callback', []);
if nargin && ischar(varargin{1})
gui_State.gui_Callback = str2func(varargin{1});
end
if nargout
[varargout{1:nargout}] = gui_mainfcn(gui_State, varargin{:});
else
gui_mainfcn(gui_State, varargin{:});
end
% End initialization code - DO NOT EDIT
% --- Executes just before untitled is made visible.
function untitled_OpeningFcn(hObject, eventdata, handles, varargin)
% This function has no output args, see OutputFcn.
% hObject handle to figure
% eventdata reserved - to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% varargin command line arguments to untitled (see VARARGIN)
% Choose default command line output for untitled
handles.output = hObject;
% Update handles structure
guidata(hObject, handles);
% UIWAIT makes untitled wait for user response (see UIRESUME)
% uiwait(handles.figure1);
global t;
% 创建 timer 对象对象的代码
timePeriod = 1.0;
t = timer('TimerFcn',{@timerCallback, handles.axes1}, 'ExecutionMode', 'fixedDelay', 'Period', timePeriod);
% 启动定时器
start(t);
function timerCallback(~, ~, axestemp)
mypic =LoadMyPic('图片\海绵宝宝.jpg');
axes(axestemp);
%这一句只运行一次!!就注释掉
%figure('visible','off');
imshow(mypic);
% --- Outputs from this function are returned to the command line.
function varargout = untitled_OutputFcn(hObject, eventdata, handles)
% varargout cell array for returning output args (see VARARGOUT);
% hObject handle to figure
% eventdata reserved - to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
% Get default command line output from handles structure
varargout{1} = handles.output;
% --- Executes during object deletion, before destroying properties.
function figure1_DeleteFcn(hObject, eventdata, handles)
% hObject handle to figure1 (see GCBO)
% eventdata reserved - to be defined in a future version of MATLAB
% handles structure with handles and user data (see GUIDATA)
global t;
stop(t); delete(t);