aggr报错解决

先解决第二个问题：Could not generate .cloupe file

martian.StageException: Could not generate .cloupe file: 

表示csv文件格式有问题，使用od -c file.csv查看

• Missing new-line character at the end of file (\n)
• NonWordChars at the beginning of the file (eg: 357, 273, 277)

$od -c 14.csv
0000000 357 273 277   s   a   m   p   l   e   _   i   d   ,   m   o   l
0000020   e   c   u   l   e   _   h   5  \r  \n   1   4   ,   /   m   n
0000040   t   /   s   r   a   /   1   4   _   c   o   u   n   t   _   o
0000060   u   t   /   o   u   t   s   /   m   o   l   e   c   u   l   e
0000100   _   i   n   f   o   .   h   5  \r  \n   1   5   ,   /   m   n
0000120   t   /   s   r   a   /   1   5   _   c   o   u   n   t   _   o
0000140   u   t   /   o   u   t   s   /   m   o   l   e   c   u   l   e
0000160   _   i   n   f   o   .   h   5  \r  \n   1   6   ,   /   m   n
0000200   t   /   s   r   a   /   1   6   _   c   o   u   n   t   _   o
0000220   u   t   /   o   u   t   s   /   m   o   l   e   c   u   l   e
0000240   _   i   n   f   o   .   h   5  \r  \n   1   7   ,   /   m   n
0000260   t   /   s   r   a   /   1   7   _   c   o   u   n   t   _   o
0000300   u   t   /   o   u   t   s   /   m   o   l   e   c   u   l   e
0000320   _   i   n   f   o   .   h   5  \r  \n  \n  \n
0000334
# 第一行有BOM:357/273/277
# 每行有回车/r

表格最前多了357 273 277的字符

原因是使用Windows创建的CSV文件，多了开头的BOM，删除即可

sed -i 's/\xEF\xBB\xBF//' 14.csv #删除BOM
 od -c 14.csv
0000000   s   a   m   p   l   e   _   i   d   ,   m   o   l   e   c   u
0000020   l   e   _   h   5  \n   1   4   ,   /   m   n   t   /   s   r
0000040   a   /   1   4   _   c   o   u   n   t   _   o   u   t   /   o
0000060   u   t   s   /   m   o   l   e   c   u   l   e   _   i   n   f
0000100   o   .   h   5  \n   1   5   ,   /   m   n   t   /   s   r   a
0000120   /   1   5   _   c   o   u   n   t   _   o   u   t   /   o   u
0000140   t   s   /   m   o   l   e   c   u   l   e   _   i   n   f   o
0000160   .   h   5  \n   1   6   ,   /   m   n   t   /   s   r   a   /
0000200   1   6   _   c   o   u   n   t   _   o   u   t   /   o   u   t
0000220   s   /   m   o   l   e   c   u   l   e   _   i   n   f   o   .
0000240   h   5  \n   1   7   ,   /   m   n   t   /   s   r   a   /   1
0000260   7   _   c   o   u   n   t   _   o   u   t   /   o   u   t   s
0000300   /   m   o   l   e   c   u   l   e   _   i   n   f   o   .   h
0000320   5  \n  \n  \n      \n
0000326

检查文件

cat 14.csv
sample_id,molecule_h5
14,/mnt/sra/14_count_out/outs/molecule_info.h5
15,/mnt/sra/15_coun