错误：AttributeError: ‘Ui_Form‘ object has no attribute ‘pushButton_01‘

在一级界面创建二级界面对象，去操作二级界面组件时，有组件对象却提示：  File "F:\python\terminal\transducer_Window_Operation.py", line 87, in __init__
    self.combobox.pushButton_01.clicked.connect(self.on_PushButton_test_01)
AttributeError: 'Ui_Form' object has no attribute 'pushButton_01'

以上是在连接时显示没有这个组件对象

class transducer_Window_Operation(QMainWindow,Ui_MainWindow):
    closed = Signal()
    def __init__(self):
        super(transducer_Window_Operation,self).__init__()
        self.setupUi(self) # 一级界面设置初始化UI
        self.combobox = Ui_Form()
        print(self.combobox)
        self.widget = QtWidgets.QWidget()

        self.combobox.setupUi(self.widget) # 二级界面初始化UI

解决办法：self.combobox.setupUi(self.widget) # 二级界面初始化UI

这个界面初始化要放在调用之前，我是因为放在函数中，一直报错。