文章关键词:Fluorescein Tyramide,FITC-TSA,FITC-Tyramide,荧光素酪胺,210236-90-1
一、基本信息
【性质简介】:Fluoroscein Tyramide, as an efficient fluorescent labeling reagent, plays an indispensable role in fields such as biology and medicine. It has been widely used in immunohistochemistry (IHC), immunocytochemistry (ICC), fluorescence in situ hybridization (FISH), and multicolor FISH techniques, especially in tyramine signal amplification (TSA). In TSA technology, Fluoroscein Tyre plays a crucial role. Its working principle is based on the catalytic action of horseradish peroxidase (HRP), which significantly enhances the fluorescence signal by locally depositing multiple tyramine molecules. This process is called catalytic reporter deposition (CARD), which can effectively improve detection sensitivity and resolution, providing researchers with more accurate and refined experimental data. During the CARD process, Fluoroscein Tyre plays a crucial role. It serves as a carrier for fluorescein tyramine and can bind to adjacent tyrosine residues. Under the catalytic action of HRP, the connection between fluorescein tyramine and tyrosine residues is strengthened, thereby forming a stable fluorescent conjugated structure. This structure not only enhances the intensity of the fluorescence signal, but also improves the stability of the signal, making the experimental results more reliable.
【中文名】:荧光素酪胺
【英文名】:Fluorescein Tyramide,FITC-TSA,FITC-Tyramide
【CAS号】:210236-90-1
【化学式】:C29H21NO7
【分子量】:495.48
【结构式】:
二、详情介绍
【外观】:固体/粉末
【溶解性】:溶于大部分有机溶剂
【规格】:10mg、25mg、50mg
【纯度】:95%+
【储存条件】:本品应密封避光,储存于阴凉、干燥、通风处,避免反复冻融,储液应该立即使用
【注意事项】:试剂仅用于科学研究或者工业应用等非医疗目的,非药用,非食用!
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