质粒图谱_常用质粒检测工具

质粒图谱

Plasmids play an important role in the functional adaptation of species for different environments. Sometimes plasmids can be circular in nature. Furthermore, plasmids can appear with a copy number which means it can occur as few times as its corresponding chromosomes. Because of these and many other numerous interesting facts, the recovery of plasmids has become an interesting research topic.

质粒在物种针对不同环境的功能适应中起着重要作用。 有时质粒本质上可以是环状的。 此外，质粒可以以拷贝数出现，这意味着它可以出现与其对应染色体相同的次数。 由于这些以及许多其他有趣的事实，质粒的回收已成为有趣的研究课题。

Researchers in the field have come up with intriguing methods to address the need for plasmid recovery. Such methods include both lab culturing of bacterial samples and insilico methods.

该领域的研究人员提出了有趣的方法来解决质粒回收的需求。 这样的方法包括实验室培养细菌样品和计算机方法。

In this article, I will present two recent tools I found that uses machine learning to separate plasmids and chromosomes.

在本文中，我将介绍我发现的两个最近的工具，这些工具使用机器学习来分离质粒和染色体。

PlasFlow (2018， 核酸研究 ) (PlasFlow (2018, Nucleic Acid Research))

This tool was developed by Pawel S Krawczyk and others in 2018. The tool uses machine learning (a deep neural network) to classify contigs from assemblies into their corresponding class (chromosome or plasmid) along with its phylum. The interesting point is the classifier is tr