STAR mapping ReadAlignChunk_processChunks.cpp:115:processChunks EXITING because of FATAL ERROR in in

2019独角兽企业重金招聘Python工程师标准>>>

因为把双端的read需要判断方向，所以第一步的时候就已经用根据建库的方式，duTP建库第一条链做反向互补，在read后面加上/1，第二条链加上/2，SMART建库的时候，第二条链做反向互补加上/1，第二条链加上/2。因为做这个转化的时候在read名字后面多了一个space号，所以出现了上面类似的问题

@E00511:189:HC3TYCCXY:5:1101:3051:1959/1 

GGGCATGGGATTTACTTATTCAGCAAAGGGTTGCCTTTACCACCCAGAAGGATTTTGGAAGAGAGGCCAGAGAGATAAGATGGGCTCATGCTCAGAGGACTTTGCATGGATTACAACCACCAGACACAAAACTGTTTGGTGACAGAA

+

<F7FJFFJJJAJJJFJJJJJJJJJFAJJJJJJJJJJJJJJJJJFFFFJJJJJA<A<JJJJJJJJJJJJJJJJJJJJJJJJJFJJJJJAJJJFJJJJJJJJFAAJFJFJJJ-FJFJJJ-A-FJJJJJJJJJJJJJJJJFJJFJJJJJJ

@E00511:189:HC3TYCCXY:5:1101:3133:1959/2

GGTGTAGCAGGAACTGGACCGTCCTCCGCGAGGTCTGATAATGCCTCGATTGGTGTATTGCCTGCTAATTTGTCTTCTAATAGAGAGAGCAAGCCCATTTCAGAGAACCCGGAAGAAGAGAACGATGGAGTCCGGGATATTCATGTA

+

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转载于:https://my.oschina.net/u/3722845/blog/1573972