1.Seurat
转自:https://cloud.tencent.com/developer/article/1055892
# Initialize the Seurat object with the raw (non-normalized data). Keep all # genes expressed in >= 3 cells (~0.1% of the data). Keep all cells with at # least 200 detected genes pbmc <- CreateSeuratObject(raw.data = pbmc.data, min.cells = 3, min.genes = 200, project = "10X_PBMC")
这里的min.genes是对细胞来说的,过滤掉那些检测到基因个数少于200的细胞。保留>=200的吧。
min.cells=200,包含那些基因,至少在200个细胞里表达了。