linux下blast设计引物,下面是我设计的引物的BLAST结果，请大虾帮我解释一下。谢谢...

下面是我设计的引物的BLAST结果，请大虾帮我解释一下。我这对引物做PCR是否有问题？谢谢。Query=

(40 letters)

Database: All GenBank+EMBL+DDBJ+PDB sequences (but no EST, STS,

GSS,environmental samples or phase 0, 1 or 2 HTGS sequences)

2,862,112 sequences; 12,768,194,998 total letters

If you have any problems or questions with the results of this search please refer to the BLAST FAQs

Taxonomy reports

Distribution of 83 Blast Hits on the Query Sequence

--------------------------------------------------------------------------------

Score E

Sequences producing significant alignments: (bits) Value

gi|28315973|tpe|BN000065.1|HSA000065 TPA: Homo sapiens SMP1... 46 0.004

gi|49256907|emb|AL928711.18| Human DNA sequence from clone ... 46 0.004

gi|40733884|emb|BX855602.2| Human DNA sequence from clone X... 46 0.004

gi|21750288|dbj|AK091825.1| Homo sapiens cDNA FLJ34506 fis,... 46 0.004

gi|37665388|emb|BX649364.3| Human DNA sequence from clone R... 46 0.004

gi|34494689|emb|BX640519.2| Human DNA sequence from clone R... 46 0.004

gi|6002137|emb|AL031284.9|HS469D22 Human DNA sequence from ... 46 0.004

gi|46389547|ref|NG_003255.1| Homo sapiens Rhesus blood grou... 46 0.004

gi|2739326|emb|Z97333.1|HSRHCE567 Homo sapiens RHCE gene, (... 46 0.004

gi|4150997|emb|Z97334.1|HSRHD567 Homo sapiens RHD gene, (pa... 46 0.004

gi|9955424|dbj|AB035199.1| Homo sapiens RHCE gene, intron 6... 46 0.004

gi|9955425|dbj|AB035200.1| Homo sapiens RHD gene, intron 6,... 46 0.004

gi|20339039|emb|AL139426.16| Human DNA sequence from clone ... 46 0.004

gi|26801335|gb|AC121585.3| Mus musculus BAC clone RP23-265M... 38 0.87

gi|4176442|emb|AL022238.1|HS1042K10 Human DNA sequence from... 38 0.87

gi|38565518|gb|AY449385.1| Homo sapiens rhesus blood group ... 36 3.4

gi|38565516|gb|AY449384.1| Homo sapiens rhesus blood group ... 36 3.4

gi|38565514|gb|AY449383.1| Homo sapiens rhesus blood group ... 36 3.4

gi|38565512|gb|AY449382.1| Homo sapiens rhesus blood group ... 36 3.4

gi|38565510|gb|AY449381.1| Homo sapiens rhesus blood group ... 36 3.4

gi|20336224|ref|NM_016124.2| Homo sapiens Rhesus blood grou... 36 3.4

gi|20336226|ref|NM_016225.2| Homo sapiens Rhesus blood grou... 36 3.4

gi|55831592|gb|AC151778.1| Ornithorhynchus anatinus chromos... 36 3.4

gi|53794104|gb|AY751496.1| Homo sapiens isolate Del9+170 Rh... 36 3.4

gi|53794102|gb|AY751495.1| Homo sapiens isolate Del89+170 R... 36 3.4

gi|53794100|gb|AY751494.1| Homo sapiens isolate Del9 Rhesus... 36 3.4

gi|53794098|gb|AY751493.1| Homo sapiens isolate Del89 Rhesu... 36 3.4

gi|53794096|gb|AY751492.1| Homo sapiens isolate Del79 Rhesu... 36 3.4

gi|53794094|gb|AY751491.1| Homo sapiens isolate Del789 Rhes... 36 3.4

gi|45379256|emb|BX784036.6| Zebrafish DNA sequence from clo... 36 3.4

gi|21311250|gb|AF390114.1| Homo sapiens Rhesus blood group ... 36 3.4

gi|56562202|emb|AJ867775.1| Homo sapiens partial RHCE gene ... 36 3.4

gi|40714024|emb|BX248328.6| Zebrafish DNA sequence from clo... 36 3.4

gi|56409833|emb|AJ867387.1| Homo sapiens partial RHD gene f... 36 3.4

gi|50871786|emb|AJ704215.2| Homo sapiens partial RHD gene f... 36 3.4

gi|50657038|emb|AJ784312.1| Homo sapiens partial RHD gene f... 36 3.4

gi|50657036|emb|AJ784311.1| Homo sapiens partial RHD gene f... 36 3.4

gi|50657034|emb|AJ784310.1| Homo sapiens partial RHD gene f... 36 3.4

gi|50657032|emb|AJ784309.1| Homo sapiens partial RHD gene f... 36 3.4

gi|20503262|gb|AC116631.6| Homo sapiens BAC clone RP11-429B... 36 3.4

gi|10800053|dbj|AB049754.1| Homo sapiens RHD mRNA for Rh bl... 36 3.4

gi|38153716|emb|AJ580940.1|HSA580940 Homo sapiens partial R... 36 3.4

gi|37650533|emb|AJ585040.1| Homo sapiens partial RHD gene f... 36 3.4

gi|36046|emb|X63097.1|HSRHXIII H.sapiens mRNA for rhesus po... 36 3.4

gi|21907993|dbj|AP004975.1| Lotus corniculatus var. japonic... 36 3.4

gi|16040905|emb|AJ276015.1|HSA276015 Homo sapiens partial R... 36 3.4

gi|7018341|emb|AJ287294.1|HSA287294 Homo sapiens partial RH... 36 3.4

gi|10334531|emb|AJ299025.1|HSA299025 Homo sapiens partial R... 36 3.4

gi|18093076|gb|AC097484.3| Homo sapiens BAC clone RP11-147K... 36 3.4

gi|25573111|gb|AF510070.1| Homo sapiens Rhesus blood group ... 36 3.4

gi|25573107|gb|AF510069.1| Homo sapiens Rhesus blood group ... 36 3.4

gi|6164858|gb|AF187846.1|AF187846 Homo sapiens RhD type III... 36 3.4

gi|5911384|gb|AF177938.1|AF177938 Homo sapiens Rh blood gro... 36 3.4

gi|11128446|gb|AC084732.1|AC084732 Homo sapiens BAC clone R... 36 3.4

gi|4104640|gb|AF037626.1|AF037626 Homo sapiens truncated Rh... 36 3.4

gi|11878260|gb|AF312679.1|AF312679 Homo sapiens rhesus D ca... 36 3.4

gi|999309|gb|S78509.1|S78509 RhD [human, D--phenotype, eryt... 36 3.4

gi|693866|gb|S73913.1|S73913 Rh D=RhK562-II [human, erythro... 36 3.4

gi|546725|gb|S70174.1|S70174 RhD=Rh blood group system D-an... 36 3.4

gi|299051|gb|S57971.1|S57971 Rh polypeptide II {clone RhPII... 36 3.4

gi|36027|emb|X63094.1|HSRHII H.sapiens mRNA for rhesus poly... 36 3.4

gi|18307511|emb|AJ428455.1|HSA428455 Homo sapiens partial R... 36 3.4

gi|606991|gb|L37050.1|CHPRHC Pan troglodytes (clone 317-IIR... 36 3.4

gi|1703665|gb|S82449.1|S82449 Homo sapiens D category IIIc ... 36 3.4

gi|6759365|dbj|AB037270.1| Homo sapiens RhIVb(J) mRNA for R... 36 3.4

gi|5360898|dbj|AB018969.1| Homo sapiens RhDYO mRNA for Rh b... 36 3.4

gi|5360246|dbj|AB018968.1| Homo sapiens RhDVa(TT) mRNA for ... 36 3.4

gi|5360244|dbj|AB018967.1| Homo sapiens RhDVa(TO) mRNA for ... 36 3.4

gi|5360242|dbj|AB018966.1| Homo sapiens RhDVa(FK) mRNA for ... 36 3.4

gi|12381901|dbj|AB046420.1| Homo sapiens RhDTI mRNA for Rh ... 36 3.4

gi|337390|gb|L08429.1|HUMRHDANTI Human RhD blood group anti... 36 3.4

Alignments

>gi|28315973|tpe|BN000065.1|HSA000065 TPA: Homo sapiens SMP1 gene, RHD gene and RHCE gene

Length = 315242

Score = 46.1 bits (23), Expect = 0.004

Identities = 26/27 (96%)

Strand = Plus / Plus

Query: 14 ctccggtccctgtccttgagttctgtg 40

|||| ||||||||||||||||||||||

Sbjct: 249813 ctccagtccctgtccttgagttctgtg 249839

Score = 46.1 bits (23), Expect = 0.004

Identities = 26/27 (96%)

Strand = Plus / Minus

Query: 14 ctccggtccctgtccttgagttctgtg 40

|||| ||||||||||||||||||||||

Sbjct: 165008 ctccagtccctgtccttgagttctgtg 164982

Score = 36.2 bits (18), Expect = 3.4

Identities = 18/18 (100%)

Strand = Plus / Plus

Query: 1 tggctgggctgatctccg 18

||||||||||||||||||

Sbjct: 164689 tggctgggctgatctccg 164706

>gi|49256907|emb|AL928711.18| Human DNA sequence from clone RP11-335G20 on chromosome 1 Contains the

gene for small membrane protein 1 (SMP1), the RHD gene for

Rhesus blood group D antigen, a succinate dehydrogenase

complex subunit D integral membrane protein (SDHD)

pseudogene and a CpG island, complete sequence

Length = 105321

Score = 46.1 bits (23), Expect = 0.004

Identities = 26/27 (96%)

Strand = Plus / Minus

Query: 14 ctccggtccctgtccttgagttctgtg 40

|||| ||||||||||||||||||||||

Sbjct: 37744 ctccagtccctgtccttgagttctgtg 37718

Score = 36.2 bits (18), Expect = 3.4

Identities = 18/18 (100%)

Strand = Plus / Plus

Query: 1 tggctgggctgatctccg 18

||||||||||||||||||

Sbjct: 37425 tggctgggctgatctccg 37442

>gi|40733884|emb|BX855602.2| Human DNA sequence from clone XXyac-25BH1 on chromosome 1, complete

sequence

Length = 173910

Score = 46.1 bits (23), Expect = 0.004

Identities = 26/27 (96%)

Strand = Plus / Minus

Query: 14 ctccggtccctgtccttgagttctgtg 40

|||| ||||||||||||||||||||||

Sbjct: 27701 ctccagtccctgtccttgagttctgtg 27675

这是BLAST连接：http://www.ncbi.nlm.nih.gov/BLAST/Blast.cgi

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