Mega-序列比对

 

Aligning Sequences

 

 

In this tutorial, we will show how to create a multiple sequence alignment from protein sequence data that will be imported into the alignment editor using different methods. All of the data files used in this tutorial can be found in the MEGA\Examples\ folder (The default location for Windows users is C:\Users\UserName\Documents\MEGA7\Examples\\. The location for Mac users is $HOME/MEGA/Examples, where $HOME is the user’s home directory).

Opening an Alignment

The Alignment Explorer is the tool for building and editing multiple sequence alignments in MEGA.

Example 2.1:

Launch the Alignment Explorer by selecting the Align | Edit/Build Alignmenton the launch bar of the main MEGA window.

Select Create New Alignment and click Ok. A dialog will appear asking “Are you building a DNA or Protein sequence alignment?” Click the button labeled “DNA”.

From the Alignment Explorer main menu, select Data | Open | Retrieve sequences from File