AbMole| BODIPY 581/591 C11 (CAS号：217075-36-0)

BODIPY 581/591 C11 是一种 BODIPY 氟硼荧衍生物，具有良好的光稳定性、低荧光伪影特质。BODIPY 581/591 C11 常用于活细胞内脂质过氧化和抗氧化性能的研究，或与羟基自由基反应、检测铁死亡 (ferroptosis)。BODIPY 581/591 C11 发射波长为 591 nm (还原型)，或红移至 510 nm (氧化型)；激发波长分别为 581 nm (还原型)、500 nm (氧化型)。

1实验参考

Lipid peroxidation was analyzed by using BODIPY 581/591 C11 (BODIPY C11) (M29325, Abmole Bioscience Inc., USA) as a lipid-soluble ratiometric fluorescent indicator. BODIPY C11 exhibits good spectral separation of the oxidized and reduced forms. T1F-transfected HEK293T cells were cultured in a 1.5 mL suspension with a final concentration of 1 μM BODIPY C11 for 30 min at 37 °C. After washing and an additional 30 min incubation, cells were observed via the LSCM with the reduced dye Ex/Em = 581/591 nm and oxidized dye Ex/Em = 488/510 nm before and after applying the AMF (518 kHz, 16 kA/m) for 10 min. The ratio of oxidized dyes to reduced dyes in the selected cells was measured with ImageJ software, which indicated the level of cellular lipid peroxidation. The ratio was normalized relative to the average ratio of each single image before applying AMF. The iron ion chelator PIH was also used as a control. Besides, lipid peroxidation levels were also measured using a flow cytometer at 495/529 nm.（整理自引用文献，仅供参考）

https://pubmed.ncbi.nlm.nih.gov/38654432/

2化学性质

分子量：504.42    

分子式：C30H35BF2N2O2    

CAS号：217075-36-0    

性状：Solid    

溶解性：（25°C）    DMSO 11 mg/mL (May need ultrasonic)    

储存条件：-20°C, protect from light    

运输方式：冰袋运输，根据产品的不同，可能会有相应调整。    

3储备液配置

4参考文献

[1] Yang Jiang, et al. J Exp Clin Cancer Res. CircLRFN5 inhibits the progression of glioblastoma via PRRX2/GCH1 mediated ferroptosis

[2] Maria F Gallelli, et al. Reprod Domest Anim. Detection of lipid peroxidation in dog spermatozoa with the fluorescent dye C11-BODIPY581/591

[3] Gregor P C Drummen, et al. Free Radic Biol Med. C11-BODIPY(581/591), an oxidation-sensitive fluorescent lipid peroxidation probe: (micro)spectroscopic characterization and validation of methodology

[4] E H Pap, et al. FEBS Lett. Ratio-fluorescence microscopy of lipid oxidation in living cells using C11-BODIPY(581/591)