R语言处理FASTQ文件报错相关

setwd("E:/CTQ/plw/3w3d")##设置路径
install.packages("Biostrings")##下载包

> seqdata = readBStringSet(filepath, format=“fasta”, nrec=-1L, skip=0L, seek.first.rec=FALSE, use.names=TRUE)
Error: unexpected input in "seqdata = readBStringSet(filepath, format=“"

从CSDN复制粘贴过去之后是中文标点，重新输入英文标点。

a = readBStringSet(R1,format="fastq",nrec=-1L,skip=0L,seek.first.rec=FALSE,use.names=TRUE)
Error in readBStringSet(R1, format = "fastq", nrec = -1L, skip = 0L, seek.first.rec = FALSE,  : 
  could not find function "readBStringSet"

> library(Biostrings)##下载后需安装包

> a = readBStringSet(R1,format="fastq",nrec=-1L,skip=0L,seek.first.rec&#