使用ArchR进行ATAC-seq测序遇到的问题2

peakAnnoEnrichment

peakAnnoEnrichment

// An highlighted block
motifsUp <- peakAnnoEnrichment(
  seMarker = markerTest,
  ArchRProj = projHeme5,
  peakAnnotation = "Motif",
  cutOff = "FDR <= 0.1 & Log2FC >= 0.25"
)

问题：

// An highlighted block
Error in .local(x, na.rm, dims, ...) :    
object 'R_sparse_marginsum' not found

解决方法：

这个问题还是Seurat、SeuratObject和Matirx的版本导致的，之前的问题降了版本，但计算到peakAnnoEnrichment这一步，就报错了。参考了其他资料，需要升高版本，即：

Seurat_5.0.1
SeuratObject_5.0.1
Matrix_1.6.4

问题解决。

ArchR logging to : ArchRLogs/ArchR-peakAnnoEnrichment-31fc586071df-Date-2023-12-18_Time-13-47-01.17584.log
If there is an issue, please report to github with logFile!
2023-12-18 13:47:03.154182 : Computing Enrichments 1 of 1, 0.033 mins elapsed.
ArchR logging successful to : ArchRLogs/ArchR-peakAnnoEnrichment-31fc586071df-Date-2023-12-18_Time-13-47-01.17584.log