单细胞（Single cell RNA-seq）教程课程与github资源

单细胞（Single cell RNA-seq）教程课程与资源

1. 工具/工作流程

1.1 scverse

生命科学中单细胞组学数据的基础工具

https://scverse.org/

1.2 SimplesingleCell

使用Bioconductor对单细胞RNA-seq数据进行低水平分析的分步工作流程

https://bioconductor.org/packages/release/workflows/html/simpleSingleCell.html

2 单细胞最佳实践

2.1 论文

论文： Current best practices in single‐cell RNA‐seq analysis: a tutorial

https://www.embopress.org/doi/full/10.15252/msb.20188746

2.2 Scanpy单细胞最佳实践

https://www.sc-best-practices.org/preamble.html

3. Github课程资源

3.1 单细胞生物信息学方法笔记

github: https://github.com/YeoLab/single-cell-bioinformatics

3.2 剑桥大学单细胞RNA-seq数据分析教程

github: https://github.com/hemberg-lab/scRNA.seq.course

3.3 单细胞RNA-seq数据低水平分析的逐步工作流程

f1000 workflow paper Aaron Lun在diffHiC、GenomicInteractions和csaw杂志上发表的《单细胞RNA-seq数据低水平分析的逐步工作流程》

https://f1000research.com/articles/5-2122/v1

4. 单细胞scRNA-seq实验设计

paper: Design and Analysis of Single-Cell Sequencing Experiments

paper: Experimental design for single-cell RNA sequencing

Tutorial: guidelines for the experimental design of single-cell RNA sequencing studies