ggplot+geom_col

library(tidyverse)
df<-read.csv('ref_thinTae_wheatH03H04.csv',sep = ',',header = T)
head(df)
ggplot(df)+geom_col(aes(gsub("Chr","",chr),reas.count.ratio,fill=type),position="dodge",width=0.6,alpha=0.75)+
  geom_hline(aes(yintercept=1.5), colour="#990000", linetype="dashed")+
  theme_bw()+
  scale_fill_manual(values = c("#48D1CC", "#BA55D3"))+
  theme(
    plot.title = element_text(size=15),
    axis.text.x = element_text(size=15),
    axis.text.y = element_text(size=15),
    #axis.text.x = element_text(angle = -30,hjust = 0.5,vjust=0),
    axis.title = element_text(size = 15),
    legend.title = element_blank(),
    legend.text = element_text(size=15),
    legend.position = "bottom"
  )+
  labs(x='reference merged Chinese Spring & Thinopyrum intermedium',y='reads count ratio')+
  ggtitle('201709 reads count ratio')
ggsave("201709_H04csreads.CSthin.png",width = 10,height=7)