解锁阿尔茨海默病（AD）靶点密码，开启靶向治疗新篇章

前 言：

阿尔茨海默病（AD）是一种严重的神经退行性疾病，多发于高龄人群，主要表现为记忆、思维、分析判断、视空间辨认、情绪等障碍。从实验室到临床应用的过程充满挑战。阿尔茨海默症新型疗法的开发主要聚焦于靶向Aβ、tau、神经炎症和突触保护等。AD相关重组蛋白在推动药物发现和破译靶点相互作用中发挥重要作用。义翘神州提供AD相关的高品质药物靶点重组蛋白及抗体产品，支持AD疾病诊断研究和药物研发。

AD相关靶点：

AD的病理变化包括胞外淀粉样蛋白β（Aβ）沉积形成淀粉样斑块、Tau蛋白过度磷酸化形成神经元纤维缠结（NFT）、神经炎症、神经元丢失、突触障碍等。AD的常见生物标志物包括淀粉样前体蛋白（APP）、微管相关蛋白（Tau）、NGF、CASP9、CLU、AGER、DPYSL2、PNMT、BDNF、GSK3B、ApoE、GLP-1R、BACE1等。

这些生物标志物在AD生理和病理过程发挥着关键作用。比如，NGF、DPYSL2维持神经细胞的生长。CASP9对细胞程序性死亡（凋亡）和组织稳态至关重要。CLU、GSK3B可调节各种细胞功能。AGER在病理状态下会诱发炎症和氧化应激。PNMT与压力相关激素（如肾上腺素）的合成相关。BDNF支持神经元的存活和可塑性。BACE1与AD中Aβ的产生相关，是AD治疗的重要靶点之一。GLP1R激动剂已被证明在抗神经炎症、减轻氧化应激和神经营养等方面发挥重要作用，已成为治疗AD的潜在药物。

AD蛋白在研究中的应用：

高品质重组蛋白（如APP、Tau、ApoE等）已应用于AD药物研发，能够促进机理研究和临床前评估，从而推动AD创新疗法的开发。Gao团队利用重组GSK-3β蛋白（货号：10044-H07B）验证了GSK-3β与PKG之间的相互作用，发现Icariside II通过干扰PKG/GSK-3β/自噬轴减轻脑损伤。Endicott团队利用重组Tau蛋白（货号：10058-H07E）和蛋白酶抑制剂处理溶酶体，发现经buparlisib或pictilisib处理的小鼠肝溶酶体对CMA底物Tau的吸收增强。Sperling团队利用重组AChE蛋白（货号：50543-M08H）研究发现与laminin-1结合会导致神经元生长。Zhang团队利用重组APP或BACE1蛋白（义翘神州）研究发现与氧化石墨烯（GO）纳米颗粒的相互作用，可降低小鼠体内的Aβ水平并改善其记忆力。Vojdani团队利用重组β-NGF和BDNF蛋白（义翘神州）发现与抗Aβ-42肽抗体高度反应。

用于表面等离子体共振SPR检测

将不同浓度的PKG与GSK-3β（货号：10044-H07B，义翘神州）结合，利用表面等离子体共振（SPR）技术测定结合亲和力。Doi: 10.1111/bph.14912

用于CMA底物检测

与对照组相比，经buparlisib或pictilisib处理的小鼠肝溶酶体对CMA底物Tau（货号：10058-H07E，义翘神州）的摄取增加，GFAP磷酸化减少。Doi: 10.1083/jcb.202001031

用于免疫染色

抗 (His)6 Tag 抗体（绿色）对 (His)6 Tag E6-AChE （货号：50543-M08H，义翘神州）处理过的 R28 细胞进行免疫染色。细胞核用 DAPI（蓝色）染色。Doi:10.1371/journal.pone.0036683

用于WB检测

通过WB检测GO/BGO 与 APP或BACE1（义翘神州）的相互作用。S 和 P 分别指沉淀得到的上清液和沉淀颗粒。Doi: 10.7150/thno.50616

用于ELISA检测

通过ELISA方法测定对照组和 AD 血清中的抗体水平。针对各种蛋白质和肽的 IgG 抗体，包括 β-NGF 和 BDNF（义翘神州），都与 AD 有关。Doi: 10.1155/2018/1672568

✦义翘神州AD特色产品：

义翘神州的产品已支持多篇文章发表在国内外学术期刊，涉及到的产品有重组蛋白、抗体、ELISA试剂、基因等。

Human GLP1R Protein (His & AVI Tag) (Cat: 13944-H49H-B)

Purity: ≥95% determined by SEC-HPLC and SDS-PAGE.

**Human GSK3B Protein (His Tag) (Cat: 10044-H07B)**助力4篇文章发表

Immobilized His-GSK3B can bind to biotinylated human HG3C-CTNNB1

Anti-Clusterin Antibody, Mouse mAb (Cat: 11297-MM01)
应用：WB、ELISA、FCM、ICC/IF

Anti-ApoE Antibody, Rabbit pAb (Cat: 10817-RP02)
助力4篇文章发表，应用：ELISA、IHC-P

Immunochemical staining of human APOE in human liver.

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