药物敏感性评价指标

最新推荐文章于 2024-01-30 15:55:55 发布

JasonKQLin

最新推荐文章于 2024-01-30 15:55:55 发布

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分类专栏：基础生物学文章标签： IC50 EC50

本文链接：https://blog.csdn.net/linkequa/article/details/88221975

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本文深入探讨了药物处理细胞后的敏感性评估方法，重点介绍了AUC、IC50、EC50及最大效应水平等关键指标，并通过图表直观展示了不同指标在评估药物反应性中的作用。

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药物处理细胞后会通过一系列指标对细胞的敏感性进行评估，主要有一下几个方面：

Area Under the Curve (AUC)
Half maximal inhibitory concentration (IC50)
The concentration where the fitted curve crosses 50% in cell viability
Half maximal effective concentration (EC50)
The term half maximal effective concentration (EC50) refers to the concentration of a drug, antibody or toxicant which induces a response halfway between the baseline and maximum after a specified exposure time
Maximal effect level (Amax)
The maximal activity value reached within a model

用下面这张图可以很直观的看到这个指标：
在这里插入图片描述
The red curve and black curve achieve 50% reduction in cell viability at the same compound concentration, but the black curve achieves increased reduction in cell viability at higher compound concentrations. Both curves correspond to the same IC50 value (vertical dotted green line), while the area under the dose response curve (AUC) captures the increased sensitivity shown in the black curve. The blue curve illustrates a sample with limited maximal reduction in cell viability at high compound concentrations. The EC50 statistic would be the same for the blue and black curves (vertical dotted green line), while the AUC statistic captures the increased response of the black curve.

Reference

Jang I S, Neto E C, Guinney J, et al. Systematic assessment of analytical methods for drug sensitivity prediction from cancer cell line data[M]//Biocomputing 2014. 2014: 63-74.