求助大佬们,我用tbtools的one step mcscanx 做共线性分析时遇见这个问题,难住我这小白了!!!

===> Start Blast.... <===
D:\aåŸºå› å®¶æ— ç¡•å£«\ONE STEP MCSCANX\(after_C32c_filter)Triticum_urartu.IGDB.dna.toplevel.fa2739282865048933465.pep
为 D:\aåŸºå› å®¶æ— ç¡•å£«\ONE STEP MCSCANX\(after_C32c_filter)Triticum_urartu.IGDB.dna.toplevel.fa2739282865048933465.pep <<===>> D:\aåŸºå› å®¶æ— ç¡•å£«\ONE STEP MCSCANX\(after filter)Triticum_urartu.IGDB.dna.toplevel.fa2739282865048933465.pep 创建了硬链接
0

为 D:\aåŸºå› å®¶æ— ç¡•å£«\ONE STEP MCSCANX\(after_C32c_filter)Triticum_urartu.IGDB.dna.toplevel.fa2739282865048933465.pep <<===>> D:\aåŸºå› å®¶æ— ç¡•å£«\ONE STEP MCSCANX\(after filter)Triticum_urartu.IGDB.dna.toplevel.fa2739282865048933465.pep 创建了硬链接

makeblastdb -h
USAGE
makeblastdb [-h] [-help] [-in input_file] [-input_type type]
-dbtype molecule_type [-title database_title] [-parse_seqids]
[-hash_index] [-mask_data mask_data_files] [-mask_id mask_algo_ids]
[-mask_desc mask_algo_descriptions] [-gi_mask]
[-gi_mask_name gi_based_mask_names] [-out database_name]
[-blastdb_version version] [-max_file_sz number_of_bytes]
[-metadata_output_prefix ] [-logfile File_Name] [-taxid TaxID]
[-taxid_map TaxIDMapFile] [-oid_masks oid_masks] [-version]

DESCRIPTION
Application to create BLAST databases, version 2.15.0+

Use '-help' to print detailed descriptions of command line arguments
blastp -h
USAGE
blastp [-h] [-help] [-import_search_strategy filename]
[-export_search_strategy filename] [-task task_name] [-db database_name]
[-dbsize num_letters] [-gilist filename] [-seqidlist filename]
[-negative_gilist filename] [-negative_seqidlist filename]
[-taxids taxids] [-negative_taxids taxids] [-taxidlist filename]
[-negative_taxidlist filename] [-no_taxid_expansion] [-ipglist filename]
[-negative_ipglist filename] [-entrez_query entrez_query]
[-db_soft_mask filtering_algorithm] [-db_hard_mask filtering_algorithm]
[-subject subject_input_file] [-subject_loc range] [-query input_file]
[-out output_file] [-evalue evalue] [-word_size int_value]
[-gapopen open_penalty] [-gapextend extend_penalty]
[-qcov_hsp_perc float_value] [-max_hsps int_value]
[-xdrop_ungap float_value] [-xdrop_gap float_value]
[-xdrop_gap_final float_value] [-searchsp int_value] [-seg SEG_options]
[-soft_masking soft_masking] [-matrix matrix_name]
[-threshold float_value] [-culling_limit int_value]
[-best_hit_overhang float_value] [-best_hit_score_edge float_value]
[-subject_besthit] [-window_size int_value] [-lcase_masking]
[-query_loc range] [-parse_deflines] [-outfmt format] [-show_gis]
[-num_descriptions int_value] [-num_alignments int_value]
[-line_length line_length] [-html] [-sorthits sort_hits]
[-sorthsps sort_hsps] [-max_target_seqs num_sequences]
[-num_threads int_value] [-mt_mode int_value] [-ungapped] [-remote]
[-comp_based_stats compo] [-use_sw_tback] [-version]

DESCRIPTION
Protein-Protein BLAST 2.15.0+

Use '-help' to print detailed descriptions of command line arguments
D:\aåŸºå› å®¶æ— ç¡•å£«\ONE STEP MCSCANX\(after_filter)Triticum_dicoccoides.WEWSeq_v.1.0.dna.toplevel8418677619157789315.pep
D:\aåŸºå› å®¶æ— ç¡•å£«\ONE STEP MCSCANX\(after filter)Triticum_dicoccoides.WEWSeq_v.1.0.dna.toplevel8418677619157789315.pep
为 D:\aåŸºå› å®¶æ— ç¡•å£«\ONE STEP MCSCANX\(after_filter)Triticum_dicoccoides.WEWSeq_v.1.0.dna.toplevel8418677619157789315.pep <<===>> D:\aåŸºå› å®¶æ— ç¡•å£«\ONE STEP MCSCANX\(after filter)Triticum_dicoccoides.WEWSeq_v.1.0.dna.toplevel8418677619157789315.pep 创建了硬链接
Links Status:0
queryFile: (after_filter)Triticum_dicoccoides.WEWSeq_v.1.0.dna.toplevel8418677619157789315.pep
[cmd.exe, /c, makeblastdb -blastdb_version 4 -in (after_C32c_filter)Triticum_urartu.IGDB.dna.toplevel.fa2739282865048933465.pep -input_type fasta -dbtype prot -out (after_C32c_filter)Triticum_urartu.IGDB.dna.toplevel.fa2739282865048933465.pep.TBtoolsDB ]
sun.jnu.encoding:GBK
Charset.defaultCharset:UTF-8
[STDOUT]:
[STDOUT]:
[STDOUT]:Building a new DB, current time: 07/01/2024 17:47:16
[STDOUT]:New DB name: D:\a������� ˶ʿ\ONE STEP MCSCANX\(after_C32c_filter)Triticum_urartu.IGDB.dna.toplevel.fa2739282865048933465.pep.TBtoolsDB
[STDOUT]:New DB title: (after_C32c_filter)Triticum_urartu.IGDB.dna.toplevel.fa2739282865048933465.pep
[STDOUT]:Sequence type: Protein
[STDOUT]:Keep MBits: T
[STDOUT]:Maximum file size: 3000000000B
[STDOUT]:Adding sequences from FASTA; added 38007 sequences in 1.08618 seconds.
[STDOUT]:
[STDOUT]:
[STDERR]:BLAST Database error: No alias or index file found for protein database [D:\a�������] in search path [D:\a������� ˶ʿ\ONE STEP MCSCANX;;]
7月 01, 2024 5:47:19 ä¸‹åˆ biocjava.GUIexcutors.OneStepMCScanXGUIPanel$6$1 run
严é‡: null
java.lang.InterruptedException: Something Error...
at biocjava.bioDoer.BLAST.CompareTwoSeqSet.compare(CompareTwoSeqSet.java:466)
at biocjava.bioIO.BioSoftPipeServer.OneStepMCScanX.process(OneStepMCScanX.java:407)
at biocjava.GUIexcutors.OneStepMCScanXGUIPanel$6$1.run(OneStepMCScanXGUIPanel.java:208)

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