参考http://bowtie-bio.sourceforge.net/bowtie2/manual.shtml
Bowtie: 25-50 nucleotides
Bowtie 2:50-1000 nucleotides
1. 建立索引
$ bowtie2-build file.fasta file
2. 比对
$ bowtie2 -p 4 -x file -1 reads_1.fq -2 reads_2.fq -S eg2.sam (双端) --un unalingedpairends (输出未比对上的序列)
$ bowtie2 -p 4 -x file -U reads_1.fq -S eg1.sam (单端)