概念介绍
Read 读段
Read 中文翻译: 读段,来自测序仪的raw data
一个Read 可能由多个片段组成, Read的索引是测序时的顺序
Sequencing quality 测序质量
测序仪在测序的时候,每次测出来的结果可能都不一样(仪器误差 序列长度等各方面因素), 所以往往需要多测几次,最后开决定是哪一个碱基。
Phred_Figure_1.jpg
Sequencing quality 是度量测序仪测序质量的指标。
测序质量指标有很多种 , 最常用的的是Phred quality score。
Sequencing quality metrics can provide important information
about the accuracy of each step in this process, including library
preparation, base calling, read alignment, and variant calling. Base
calling accuracy, measured by the Phred quality score (Q score), is the
most common metric used to assess the accuracy of a sequencing
platform. It indica