Histone deacetylase (HDAC) activity assay
Sample type
cell extracts
people
Product overview
A histone deaminase (HDAC) activity detection reagent was used to detect HDAC activity in the solution
HDAC activity analysis reagent is mainly used to evaluate HDAC inhibitors with HDAC components In addition, when a specific dose of HDAC inhibitor, such as a inhibitor, is used, Reagents can be used to analyze HDAC activity of any vegetative cell, cell or tissue
The activity determination scheme of HDAC is based on acetylated peptides related to AMC AMC is a fluorescent dye that fluoresces when it binds to peptides When HDAC causes peptide acetylation, it is easily dismembered by enzymes (developer components). A free AMC was subsequently released, which can be measured by a fluorescent micropore reader (ex / EM 355 / 460 nm).
It has been proved that HDAC analysis can detect the activity of HDAC family and at least detect the division of human or animal cells or HDAC fractions, The analysis shows that the reaction of the sample is linear. This analysis can be used to monitor the decontamination of HDAC or detect the presence of HDAC in cells
say
This application set includes:
Monitor HDACs cleaning, including HDAC1, 2, 3 and 8.
Choose HDACs inhibitor or activator
Test the effect of drugs on HDACs
Background information of HDACs
Proteomic degassing enzymes (HDAC) are a class of lysine acetylases at the N-terminal of major proteomes (H2A, H2B, H3 and H4), Enables the proteome to package DNA more tightly.
According to functional equivalence and different DNA sequences, HDAC proteins are divided into four categories (class I, class II A, class II IB, class III and class IV).
Class I, class II A and class II IIB are considered as "classics" of HDAC, and their activity is inhibited by antibacterial inhibitor A (TSA). The third class is a subclass of NAD + dependent proteins (sirtuins), Not applicable to TSA. The fourth category itself is considered to be an atypical category, which is only based on the analogy with other DNA sequence types.
platform:
Functional Assay service Tumor cell line proliferation assay Kinase Functional Screening Other Enzymatic Functional Assay Nuclear Receptor Target Functional Assay Cell Apoptosis Testing CACO-2 Cell Permeability Model High Throughput Screening of Compounds Gene Expression Analysis Protein Expression Analysis Selectivity/Safety Evaluation Services Tumor Cells Panel Nuclear Receptor Panel Kinase Panel Drug Safety Assessment of Hepatotoxicity Customization Service Stable Cell Line Generation Protein Interaction System Development Evaluation of Total Protein or Phosphorylated Protein Enzymatic Target Assay Development Popular Drug Targets PDEs Series EGFR Series CDKs Series PARPS Series KRAS Series
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