1.HiC PRO–分析部分:
1.1. 安装HiC PRO依赖的软件。例如所使用的HiC-PRO3.1.0版本的依赖软件和版本如下:
序列比对软件:bowtie2;
序列处理软件:samtools;
环境管理软件:conda或docker二选一;
任务调度软件:TORQUE或SGE 或 SLURM三选一;
R4.0及以上;
python3.7及以上;
1.2. 下载并解压HiC-PRO3.1.0,按照以下步骤创建环境、配置和安装HiC-PRO。
19-01-15
Nicolas Servant
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HiC-Pro Installation
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All dependencies will be checked during installation, and installed if
possible. The installation process will generate a config-system.txt
file which defines all paths to HiC-Pro dependencies. Please, do not
edit this file !
Install Dependencies
Dependencies are checked during installation. A couple of tools such
asbowtie2
andsamtools
(>=1.9) can be automatically installed if
not detected. Others have to be installed before starting the
installation :
- R (http://www.r-project.org/) with the following packages
— ggplot2 (>2.2.1)
— RColorBrewer
— grid- Python (>3.7) libraries :
<
— bx-python (>=0.8.8) - https://pypi.python.org/pypi/bx-python
— numpy (>=1.18.1) - http://www.scipy.org/scipylib/download.html
— scipy (>=1.4.1) - http://www.scipy.org/scipylib/download.html
— pysam (>=0.15.4) - https://github.com/pysam-developers/pysam
— argparse