derivate_gauss (Image, DerivGauss, 4, 'xx')
mean_image (DerivGauss, ImageMean, 2, 50)
threshold (ImageMean, Regions, -0.5, -0.15087)
connection (Regions, ConnectedRegions)
select_shape_std (ConnectedRegions, SelectedRegions, 'max_area', 70)
edges_sub_pix (Image, Edges, 'canny',28, 20,20)
select_contours_xld (Edges, SelectedContours, 'closed' , 0.5, 0, -0.5, 0.5)
select_contours_xld (SelectedContours, SelectedContours1, 'contour_length' , 8000, 9000, -0.5, 0.5)