关于荧光共定位分析

What is colocalization?

Suppose you are given some images by a colleague, or have some images of your own, and you want to measure the amount of colocalisation between two of the dyes or stains in the images. First you have to define what you mean by colocalisation, and that is not trivial. For one place to start reading about colocalisation and for how to correctly capture quantitative fluorescence microscopy images suitable for colocalisation analysis, look here: Image Processing Courses at BioDIP, Dresden. A recent review isby Dunn in 2011.

A sample dataset

Open this sample data file colocsample1bRGB_BG.tif

Then use the "Image-Color-Split Channels" menu command to get a separate z stack for the 2 dyes (you can throw the blue one away!).

If you like, you can change the look up tables of the images (LUTs) so one is "green" and one is "magenta". Of course the colors here are always false. These false colors are only useful to tell which channel is which.