Pfam是一个广泛使用的蛋白家族数据库，提供手动编辑的家族信息，覆盖了近80%的UniProtKB序列。文章介绍了Pfam的内容更新，包括基于不同代表蛋白数据集的家族对齐和新的交互式DNA搜索界面。此外，详细阐述了如何使用PfamScan工具进行本地化蛋白结构域注释，包括数据库下载、安装、建索引和运行分析的步骤及参数解释。
Pfam: the protein families database11.501Nucleic Acids Res . 2014 Jan;42(Database issue):D222-30. doi: 10.1093/nar/gkt1223. Epub 2013 Nov 27.
Abstract
Pfam, available via servers in the UK (http://pfam.sanger.ac.uk/) and the USA (http://pfam.janelia.org/), is a widely used database of protein families, containing 14 831 manually curated entries in the current release, version 27.0. Since the last update article 2 years ago, we have generated 1182 new families and maintained sequence coverage of the UniProt Knowledgebase (UniProtKB) at nearly 80%, despite a 50% increase in the size of the underlying sequence database. Since our 2012 article describing Pfam, we have also undertaken a comprehensive review of the features that are provided by Pfam over and above the basic family data. For each feature, we determined the relevance, computational burden, usage statistics and the functionality of the feature in a website context. As a conse
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