Schlemm管内荧光素钠造影术/活化PⅡb/Ⅲa配体MRI显影剂/肝素标记超顺磁氧化铁粒子(Hep-SPIO)

Schlemm管内荧光素钠造影术/活化PⅡb/Ⅲa配体MRI显影剂/肝素标记超顺磁氧化铁粒子(Hep-SPIO)

操作流程

1. 造影剂的配制：使用生理盐水将10%荧光素钠注射液稀释到浓度为0.15%，使用10 ml 注射器抽取荧光素钠稀释液8~10 ml 备用。

 

2. Schlemm 管定位：制作5 mm×5 mm，1/4 巩膜厚度的抛物线形的浅层巩膜瓣，向前剖至角膜缘内1 mm。在浅层巩膜瓣边缘内0.5 mm 处，制作4 mm×4 mm，2/3 巩膜厚度的深层巩膜瓣，以透见下方黑紫色脉络膜为佳，暴露Schlemm 管断端，去除外壁，开放Schlemm 管两端切口。

 

3. 做前房穿刺口，放出适量房水，测量并记录眼压，使眼压维持在12~18 mmHg 范围内。若眼压过低，可通过穿刺口注入适量生理盐水。

 

4. 造影剂注入Schlemm 管：将装有0.15%荧光素钠的10 ml 注射器连接至微导管末端，推注荧光素钠使其充满整个微导管。操作时应避免荧光素钠流出而污染手术区。将微导管插入Schlemm 管内3mm，确认导管未迷路入前房或脉络膜上腔后，将手术显微镜光源处的滤光片调至钴蓝滤光片，并开始采集图像。助手以恒定的力量推注装有荧光素钠造影剂的10 ml 注射器，当刚刚有荧光素钠造影剂进入前房后，主刀者边将微导管匀速送入Schlemm 管内，助手边推注造影剂，直至微导管从对侧Schlemm管断端穿出。

 

5. 按Schlemm 管成形术或微导管引导的360度小梁切开术操作规范完成后续手术操作。手术结束前，如果前房内有荧光素钠积存，应将前房内荧光素钠冲洗干净。

 

1. 分析：记录术中眼压，根据术中录像记录有荧光素钠充盈的远端房水流出通道（图1）的位置及范围。
2. 术中Schlemm 管内荧光素钠造影显示远端房水流出通道。A～C 显示远端房水流出通道动态充盈的过程。箭头指示充盈荧光素钠的远端房水流出通道，红色区域代表微导管头端的位置

 

活体荧光造影剂

Sulfo-CY7(Cyanine7)NHSester

Sulfo-Cyanine7.5NHSester,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics

 

sulfo-CY7(Cyanine7)maleimide

sulfo-Cyanine7.5maleimide,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics

 

Sulfo-CY7(Cyanine7)dicarboxylicacid

Sulfo-Cyanine7.5dicarboxylicacid,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics

 

Sulfo-CY7(Cyanine7)carboxylicacid

Sulfo-Cyanine7.5carboxylicacid,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics

 

Sulfo-CY7(Cyanine7)azide

Sulfo-Cyanine7.5azide,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics

 

Sulfo-CY7(Cyanine7)alkyne

Sulfo-Cyanine7.5alkyne,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics

 

Sulfo-CY7(Cyanine7)tetrazine

Sulfo-Cyanine7tetrazine,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics

 

Sulfo-CY7(Cyanine7)NHSester

Sulfo-Cyanine7NHSester,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics

 

Sulfo-CY7(Cyanine7)maleimide

Sulfo-Cyanine7maleimide,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics

 

sulfo-CY7(Cyanine7)dicarboxylicacid

sulfo-Cyanine7dicarboxylicacid,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics

 

Sulfo-CY7(Cyanine7)carboxylicacid

Sulfo-Cyanine7carboxylicacid,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics

 

Sulfo-CY7(Cyanine7)bis-NHSester

Sulfo-Cyanine7bis-NHSester,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics

 

Sulfo-CY7(Cyanine7)azide

Sulfo-Cyanine7azide,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics

 

Sulfo-CY7(Cyanine7)amine

Sulfo-Cyanine7amine,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics

 

Sulfo-CY7(Cyanine7)alkyne

Sulfo-Cyanine7alkyne,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics

 

CY7(Cyanine7)tetrazine

Cyanine7.5tetrazine,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics

 

CY7(Cyanine7)NHSester

Cyanine7.5NHSester,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics

 

CY7(Cyanine7)maleimide

Cyanine7.5maleimide,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics

 

CY7(Cyanine7)hydrazide

Cyanine7.5hydrazide,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics

 

CY7(Cyanine7)carboxylicacid

Cyanine7.5carboxylicacid,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics

 

CY7(Cyanine7)azide

Cyanine7.5azide,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecularbiologyanddiagnostics

 

CY7(Cyanine7)azide,10mM/DMSO

Cyanine7.5azide,10mM/DMSO,Comparisonofcyanine(Cy)dyesusedinlifescienceaslabelsformolecul

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