whole-genome-sequencing Data Analysis 学习笔记1 基本概念

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探索全基因组测序数据
1.参考文章
Systematic Characteristic Exploration of the Chimeras Generated in Multiple Displacement Amplification through Next Generation Sequencing Data Reanalysis
注释：
Chimera (genetics)
A genetic chimerism or chimera (also spelled chimaera) is a single organism composed of cells from different zygotes. This can result in male and female organs, two blood types, or subtle variations in form.[1] Animal chimeras are produced by the merger of multiple fertilized eggs. In plant chimeras, however, the distinct types of tissue may originate from the same zygote, and the difference is often due to mutation during ordinary cell division. Normally, genetic chimerism is not visible on casual inspection; however, it has been detected in the course of proving parentage.[2]
Another way that chimerism can occur in animals is by organ transplantation, giving one individual tissues that developed from two genomes. For example, a bone marrow transplant can change someone’s blood type.
[1]Norton, Aaron; Ozzie Zehner (2008). “Which Half Is Mommy?: Tetragametic Chimerism and Trans-Subjectivity”. Women’s Studies Quarterly. Fall/Winter: 106–127.
[2] Friedman, Lauren. “The Stranger-Than-Fiction Story Of A Woman Who Was Her Own Twin”. Retrieved 4 August 2014.

Method

We finished data mining towards a series of Next Generation Sequencing (NGS) reads
We established a bioinformatics pipeline based on subsection alignment strategy to discover all the chimeras