自用 学习BCR 免疫组化

今天所学：

用miniconda下载了pRESTO 打算用Immcantation 来进行免疫组库的分析

(base) C:\Users\XXXX>python D:\programdata\Scripts\FilterSeq.py quality
usage: FilterSeq.py quality [--version] [-h] -s SEQ_FILES [SEQ_FILES ...]
                            [-o OUT_FILES [OUT_FILES ...]] [--outdir OUT_DIR]
                            [--outname OUT_NAME] [--log LOG_FILE] [--failed]
                            [--fasta] [--nproc NPROC] [-q MIN_QUAL] [--inner]
FilterSeq.py quality: error: the following arguments are required: -s

(base) C:\Users\XXXX>python D:\programdata\Scripts\FilterSeq.py quality -s SRR17379068.fastq -q 20 --outname M1 --log FS.log
ERROR> Sequence file SRR17379068.fastq does not exist.

卡在了如何读取fastq的文件 并且调用 quality 明天再琢磨