品牌:MedChemExpress (MCE)
纯度:Greater than 95% as determined by reducing SDS-PAGE
分子式:Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4 or 50 mM Tris-HCL, 200 mM NaCL, 500 mM arginine, pH 8.0.
分子量:Approximately 17.0 kD
存储条件:在 -20°C 下可保存 2 年。复溶后,在 4°C 下可稳定保存 1 周,在 -20°C 下可稳定保存更长时间(含载体蛋白)。建议将等分试样在 -20°C 或 -80°C 下冷冻以延长保存时间。
运输条件:美国大陆的室温;其他地区可能有所不同。
产品活性:IL-17A 是一种同型二聚体细胞因子,在宿主防御机制中对许多细菌和真菌病原体以及过敏和自身免疫反应起着关键作用。IL-17A 诱导抗菌肽、细胞因子、趋化因子和基质金属蛋白酶的产生。IL-17A Protein, Human 是一种重组人 IL-17A 蛋白,在 E. coli 中表达,由 137 个氨基酸组成(I19-A155)。
生物活性:通过其诱导NIH-3T3小鼠胚胎成纤维细胞分泌IL-6的能力来测量,ED50为1-7 ng/mL。
参考详情:www.medchemexpress.com/recombinant-proteins/il-17a-protein-human.html
参考文献:
[1]. Chen K, et al. Interluekin-17A (IL17A). Gene. 2017 May 30;614:8-14.
[2]. Iwakura Y, et al. The roles of IL-17A in inflammatory immune responses and host defense against pathogens. Immunol Rev. 2008 Dec;226:57-79.
[3]. Cua DJ, et al. Innate IL-17-producing cells: the sentinels of the immune system. Nat Rev Immunol. 2010 Jul;10(7):479-89.
[4]. Wright JF, et al. The human IL-17F/IL-17A heterodimeric cytokine signals through the IL-17RA/IL-17RC receptor complex. J Immunol. 2008 Aug 15;181(4):2799-805.
[5]. Kinoshita H, et al. Cytokine milieu modulates release of thymic stromal lymphopoietin from human keratinocytes stimulated with double-stranded RNA. J Allergy Clin Immunol. 2009 Jan;123(1):179-86.
[6]. Henness S, et al. IL-17A acts via p38 MAPK to increase stability of TNF-alpha-induced IL-8 mRNA in human ASM. Am J Physiol Lung Cell Mol Physiol. 2006 Jun;290(6):L1283-90.