与之对应的是single cell RNA-Seq,后面也会有类似文章。
参考:https://github.com/xuzhougeng/Learn-Bioinformatics/
资料:RNA-seq Data Analysis-A Practical Approach(2015)
Bioinformatic Data Skill
A survey of best practices for RNA-seq data analysis
Detecting differential usage of exons from RNA-seq data
转录组入门(1): 工作环境准备
miniconda2
cd src wget https://repo.continuum.io/miniconda/Miniconda2-latest-Linux-x86_64.sh bash Miniconda2-latest-Linux-x86_64.sh
conda config --add channels https://mirrors.tuna.tsinghua.edu.cn/anaconda/pkgs/free/ conda config --add channels https://mirrors.tuna.tsinghua.edu.cn/anaconda/cloud/conda-forge/ conda config --add channels https://mirrors.tuna.tsinghua.edu.cn/anaconda/cloud/msys2/ conda config --add channels bioconda conda config --set show_channel_urls yes
在里面安装各种工具
conda create -n biostar sra-tools fastqc hisat2 samtools htseq
转录组入门(2):读文章拿到测序数据
AKAP95 regulates splicing through scaffolding RNAs and RNA processing factors. Nat Commun 2016 Nov 8;7:13347. PMID: 27824034
GSE81916,https://www.ncbi.nlm.nih.gov/geo/
for i