ATAC-seq技术原理简介

ATAC-seq

ATAC-seq (Assay for Transposase-Accessible Chromatin using sequencing) is a technique used in molecular biology to assess genome-wide chromatin accessibility.[1] In 2013, the technique was first described as an alternative advanced method for MNase-seq, FAIRE-Seq and DNase-Seq.[1] ATAC-seq is a faster and more sensitive analysis of the epigenome than DNase-seq or MNase-seq.[2][3][4]

ATAC-seq（使用测序法测定转座酶可及性染色质技术）是一种在分子生物学中用于评估全基因组染色质可及性的技术。 2013年，该技术首次被认为可以作为MNase-seq，FAIRE-Seq和DNase-Seq技术替代方案的更加高级方法。在表观基因组分析中，ATAC-seq比DNase-seq或MNase-seq更快
、更灵敏。

Contents

• 1Description
• 2Applications
• 3Single-cell ATAC-seq
• 4References
• 5External source

Description[edit]

ATAC-seq identifies accessible DNA regions by probing open chromatin with hyperactive mutant