- 在运行PROVEAN软件时,指定的nr数据库路径是:/data/software/blastdb/nr,给出的报错是BLAST Database error: No alias or index file found for protein database [/data/software/blastdb/nr/nr] in search path
# nr数据库下的文件
(base) [user@localhost examples]$ ll /data/software/blastdb/nr/
total 559692336
-rw-rw-rw- 1 root root 236883819668 Mar 3 04:14 nr
-rw-rw-r-- 1 user user 527272381 Mar 3 21:44 prot_fa.00.phr
-rw-rw-r-- 1 user user 20869088 Mar 3 21:44 prot_fa.00.pin
-rw-rw-r-- 1 user user 10434532 Mar 3 21:44 prot_fa.00.pog
-rw-rw-r-- 1 user user 999999663 Mar 3 21:44 prot_fa.00.psq
-rw-rw-r-- 1 user user 526521210 Mar 3 21:45 prot_fa.01.phr
-rw-rw-r-- 1 user user 20867784 Mar 3 21:45 prot_fa.01.pin
-rw-rw-r-- 1 user user 10433880 Mar 3 21:45 prot_fa.01.pog
-rw-rw-r-- 1 user user 999999510 Mar 3 21:45 prot_fa.01.psq
-rw-rw-r-- 1 user user 526400446 Mar 3 21:46 prot_fa.02.phr
-rw-rw-r-- 1 user user 20891984 Mar 3 21:46 prot_fa.02.pin
-rw-rw-r-- 1 user user 10445980 Mar 3 21:46 prot_fa.02.pog
-rw-rw-r-- 1 user user 999999932 Mar 3 21:46 prot_fa.02.psq
-rw-rw-r-- 1 user user 526061308 Mar 3 21:47 prot_fa.03.phr
-rw-rw-r-- 1 user user 20859688 Mar 3 21:47 prot_fa.03.pin
把provean.sh中BLAST_DB路径改为/data/software/blastdb/nr/prot_fa即可
- blastp命令运行
blastp -query P04637.fasta -db /data/software/pdb/PDB -outfmt 6 -num_threads 12