跟着Cell学单细胞转录组分析(十四):细胞比例柱状图---连线堆叠柱状图

之前做单细胞比例图的时候做过堆叠柱状图，（跟着Cell学单细胞转录组分析(六):细胞比例计算及可视化），看那篇Cell文章封面也给我一个启发，我们做的柱状图每个样本是一个单独的柱子，如果有很多细胞类型，从左到右看起来很费劲，那么如果把相同的细胞类型用线连起来的话，看起来就方便多了，包括不同组中细胞比例变化一目了然。

一般的做法如下：

table(scedata$orig.ident)#查看各组细胞数
prop.table(table(Idents(scedata)))
table(Idents(scedata), scedata$orig.ident)#各组不同细胞群细胞数
Cellratio <- prop.table(table(Idents(scedata), scedata$orig.ident), margin = 2)#计算各组样本不同细胞群比例
Cellratio <- as.data.frame(Cellratio)

allcolour=c("#DC143C","#0000FF","#20B2AA","#FFA500","#9370DB","#98FB98","#F08080","#1E90FF","#7CFC00","#FFFF00",
            "#808000","#FF00FF","#FA8072","#7B68EE","#9400D3","#800080","#A0522D","#D2B48C","#D2691E","#87CEEB","#40E0D0","#5F9EA0",
            "#FF1493","#0000CD","#008B8B","#FFE4B5","#8A2BE2","#228B22","#E9967A","#4682B4","#32CD32","#F0E68C","#FFFFE0","#EE82EE",
            "#FF6347","#6A5ACD","#9932CC","#8B008B","#8B4513","#DEB887")
library(ggplot2)
ggplot(Cellratio) + 
  geom_bar(aes(x =Var2, y= Freq, fill = Var1),stat = "identity",width = 0.7,size = 0.5,colour = '#222222')+ 
  theme_classic() +
  labs(x='Sample',y = 'Ratio')+
  scale_fill_manual(values = allcolour)+
  theme(panel.border = element_rect(fill=NA,color="black", size=0.5, linetype="solid"))

使用连线连接：

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