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一、构建bwa的基因组索引文件1、一步到位下载hg19基因组文件wget -c ftp://gsapubftp-anonymous@ftp.broadinstitute.org/bundle/hg19/ucsc.hg19*放入服务器bwa index [ –p prefix ] [ –a algoType ] <in.db.fasta>#官网提供的命令bwa index -a bwtsw hg19.fa#实际操作最后生成文件：hg19.fa.amb、hg19.fa.ann、hg

bwa_wrap hg19.fa OUTdir3/index-1_2.valid.fastq 1 OUTdir3/index-1_2.valid.sam 1Running BWA on trimmed reads ...bwa aln -t 1 hg19.fa OUTdir3/index-1_2.valid.fastq > OUTdir3/index-1_2.valid.fastq.sai; bwa samse hg19.fa OUTdir3/index-1_2.valid.fastq.sai O

#### MISO into SQL database interface##import osimport sysimport timeimport zipfileimport sqlite3import shutilimport fnmatchimport globimport StringIOimport misopyimport misopy.misc_utils as misc_utilsimport misopy.miso_utils as miso_...

1、安装python和yum系统版本：CentOS Linux release 7.3.1611 (Core)一定要选对应自己系统版本的文件！！ http://vault.centos.org/，进入网站，选择自己系统对应的版本，进入OS文件夹　　选择系统对应的位数，32位选i386，64位选择x86_64进入Packages文件夹，进去之后你会看到很多rpm文件下载如下文件：python-2.6.6-66.el6_8.x86_64.rpmpython-devel-2.6.6-66

(base) [root@node01 gaoj]# misoTraceback (most recent call last): File "/usr/bin/miso", line 5, in <module> from pkg_resources import load_entry_pointImportError: No module named pkg_resources(base) [root@node01 gaoj]# pip install pkg_resour

Install ChIA-PET2 in /root/bin/ChIA-PET2_0.9.3 ...(base) [root@node01 ChIA-PET2-0.9.3]# (base) [root@node01 ChIA-PET2-0.9.3]# (base) [root@node01 ChIA-PET2-0.9.3]# (base) [root@node01 ChIA-PET2-0.9.3]# (base) [root@node01 ChIA-PET2-0.9.3]# (base) [ro

下载python2.7版本wget https://www.python.org/ftp/python/2.7.18/Python-2.7.18.tgz解压tgz包tar -zxvf Python-2.7.18.tgz把python移到/usr/local下面mv Python-2.7.18.tgz /usr/local删除旧版本的python依赖ll /usr/bin | grep pythonrm -rf /usr/bin/python进入python目录cd /usr/l.

解决sudo 时赋予当前账户root权限（可以修改系统文件），gedit是一个编辑器，可以换成别的编辑器，如gvimsudo gedit ~/.bashrc第二步：添加到最后即可export PATH=$PATH:/root/anaconda3/bin/condaexport PATH=$PATH:/usr/bin/yum第三步：更新.bashrc文件source ~/.bashrc...

报错 File "/bin/yum", line 30 except KeyboardInterrupt, e: ^ SyntaxError: invalid syntax解决：linux安装python2.71、下载pythonwget https://www.python.org/ftp/python/2.7.16/Python-2.7.16.tgz2、解压、编译安装（依次执行以下5条命令）#tar -zxvf Pytho