GWAS相关的曼哈顿图-SNP密度图

最新推荐文章于 2024-04-24 15:25:33 发布

小孔乘象k

最新推荐文章于 2024-04-24 15:25:33 发布

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分类专栏： R与生物信息文章标签： ggplot 经验分享其他

原文链接：https://github.com/YinLiLin/CMplot

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R包 CMplot

官网链接
https://github.com/YinLiLin/CMplot

A high-quality drawing tool designed for Manhattan plot of genomic analysis

Installation

CMplot is available on CRAN, so it can be installed with the following R code:

> install.packages("CMplot")
> library("CMplot")

# if you want to use the latest version on GitHub:
> source("https://raw.githubusercontent.com/YinLiLin/CMplot/master/R/CMplot.r")

There are two example datasets attached in CMplot, users can export and view the details by following R code:

> data(pig60K)   #calculated p-values by MLM
> data(cattle50K)   #calculated SNP effects by rrblup
> head(pig60K)

          SNP Chromosome Position    trait1     trait2     trait3
1 ALGA0000009          1    52297 0.7738187 0.51194318 0.51194318
2 ALGA0000014          1    79763 0.7738187 0.51194318 0.51194318
3 ALGA0000021          1   209568 0.7583016 0.98405289 0.98405289
4 ALGA0000022          1   292758 0.7200305 0.48887140 0.48887140
5 ALGA0000046          1   747831 0.9736840 0.22096836 0.22096836
6 ALGA0000047          1   761957 0.9174565 0.05753712 0.05753712

> head(cattle50K)

   SNP chr    pos Somatic cell score  Milk yield Fat percentage
1 SNP1   1  59082        0.000244361 0.000484255    0.001379210
2 SNP2   1 118164        0.000532272 0.000039800    0.000598951
3 SNP3   1 177246        0.001633058 0.000311645    0.000279427
4 SNP4   1 236328        0.001412865 0.000909370    0.001040161
5 SNP5   1 295410        0.000090700 0.002202973    0.000351394
6 SNP6   1 354493        0.000110681 0.000342628    0.000105792

As the example datasets, the first three columns are names, chromosome, position of SNPs respectively, the rest of columns are the pvalues of GWAS or effects of GS/GP for traits, the number of traits is unlimited.
Note: if plotting SNP_Density, only the first three columns are needed.

Now CMplot could handle not only Genome-wide association study results, but also SNP effects, Fst, tajima’s D and so on.

Total 50~ parameters are available in CMplot, typing ?CMplotcan get the detail function of all parameters.

Citation

Please cite the website https://github.com/YinLiLin/R-CMplot

SNP-density plot

> CMplot(pig60K,type="p",plot.type="d",bin.size=1e6,chr.den.col=c("darkgreen", "yellow", "red"),file="jpg",memo="",dpi=300,
    file.output=TRUE,verbose=TRUE,width=9,height=6)
# users can personally set the windowsize and the min/max of legend by:
# bin.size=1e6
# bin.range=c(min, max)
# memo: add a character to the output file name
# chr.labels: change the chromosome names

在这里插入图片描述

Circular-Manhattan plot

(1) Genome-wide association study(GWAS)

> CMplot(pig60K,type="p",plot.type="c",chr.labels=paste("Chr",c(1:18,"X","Y"),sep=""),r=0.4,cir.legend=TRUE,
        outward=FALSE,cir.legend.col="black",cir.chr.h=1.3,chr.den.col="black",file="jpg",
        memo="",dpi=300,file.output=TRUE,verbose=TRUE,width=10,height=10)

在这里插入图片描述

> CMplot(pig60K,type="p",plot.type="c",r=0.4,col=c("grey30","grey60"),chr.labels=paste("Chr",c(1:18,"X","Y"),sep=""),
      threshold=c(1e-6,1e-4),cir.chr.h=1.5,amplify=TRUE,threshold.lty=c(1,2),threshold.col=c("red",
      "blue"),signal.line=1,signal.col=c("red","green"),chr.den.col=c("darkgreen","yellow","red"),
      bin.size=1e6,outward=FALSE,file="jpg",memo="",dpi=300,file.output=TRUE,verbose=TRUE,width=10,height=10)

#Note:
1. if signal.line=NULL, the lines that crosse circles won't be added.
2. if the length of parameter 'chr.den.col' is not equal to 1, SNP density that counts 
   the number of SNP within given size('bin.size') will be plotted around the circle.

在这里插入图片描述

(2) Genomic Selection/Prediction(GS/GP)

> CMplot(cattle50K,type="p",plot.type="c",LOG10=FALSE,outward=TRUE,col=matrix(c("#4DAF4A",NA,NA,"dodgerblue4",
         "deepskyblue",NA,"dodgerblue1", "olivedrab3", "darkgoldenrod1"), nrow=3, byrow=TRUE),
         chr.labels=paste("Chr",c(1:29),sep=""),threshold=NULL,r=1.2,cir.chr.h=1.5,cir.legend.cex=0.5,
         cir.band=1,file="jpg", memo="",dpi=300,chr.den.col="black",file.output=TRUE,verbose=TRUE,
         width=10,height=10)
        
#Note: parameter 'col' can be either vector or matrix, if a matrix, each trait can be plotted in different colors.

在这里插入图片描述

Single_track Rectangular-Manhattan plot

Genome-wide association study(GWAS)

> CMplot(pig60K,type="p",plot.type="m",LOG10=TRUE,threshold=NULL,file="jpg",memo="",dpi=300,
    file.output=TRUE,verbose=TRUE,width=14,height=6,chr.labels.angle=45)
# 'chr.labels.angle': adjust the angle of labels of x-axis (-90 < chr.labels.angle < 90).

在这里插入图片描述

Amplify signals on pch, cex and col

> CMplot(pig60K, plot.type="m", col=c("grey30","grey60"), LOG10=TRUE, ylim=c(2,12), threshold=c(1e-6,1e-4),
        threshold.lty=c(1,2), threshold.lwd=c(1,1), threshold.col=c("black","grey"), amplify=TRUE,
        chr.den.col=NULL, signal.col=c("red","green"), signal.cex=c(1.5,1.5),signal.pch=c(19,19),
        file="jpg",memo="",dpi=300,file.output=TRUE,verbose=TRUE,width=14,height=6)

#Note: if the ylim is setted, then CMplot will only plot the points among this interval,
#       ylim can be vector or list, if it is a list, different traits can be assigned with
#       different range at y-axis.

在这里插入图片描述

Attach chromosome density on the bottom of Manhattan plot

> CMplot(pig60K, plot.type="m", LOG10=TRUE, ylim=NULL, threshold=c(1e-6,1e-4),threshold.lty=c(1,2),
        threshold.lwd=c(1,1), threshold.col=c("black","grey"), amplify=TRUE,bin.size=1e6,
        chr.den.col=c("darkgreen", "yellow", "red"),signal.col=c("red","green"),signal.cex=c(1.5,1.5),
        signal.pch=c(19,19),file="jpg",memo="",dpi=300,file.output=TRUE,verbose=TRUE,
        width=14,height=6)
        
#Note: if the length of parameter 'chr.den.col' is bigger than 1, SNP density that counts 
   the number of SNP within given size('bin.size') will be plotted.

在这里插入图片描述

Highlight a group of SNPs on pch, cex, type, and col

> signal <- pig60K$Position[which.min(pig60K$trait2)]
> SNPs <- pig60K$SNP[pig60K$Chromosome==13 & 
        pig60K$Position<(signal+1000000)&pig60K$Position>(signal-1000000)]
> CMplot(pig60K, plot.type="m",LOG10=TRUE,col=c("grey30","grey60"),highlight=SNPs,
        highlight.col="green",highlight.cex=1,highlight.pch=19,file="jpg",memo="",
        chr.border=TRUE,dpi=300,file.output=TRUE,verbose=TRUE,width=14,height=6)
#Note:
'highlight' could be vector or list, if it is a vector, all traits will use the same highlighted SNPs index, 
if it is a list, the length of the list should equal to the number of traits.
highlight.col, highlight.cex, highlight.pch can be value or vector, if its length equals to the length of highlighted SNPs,
each SNPs have its special colour, size and shape.

> SNPs <-  pig60K[pig60K$trait2 < 1e-4, 1]
> CMplot(pig60K,type="h",plot.type="m",LOG10=TRUE,highlight=SNPs,highlight.type="p",
        highlight.col=NULL,highlight.cex=1.2,highlight.pch=19,file="jpg",memo="",
        dpi=300,file.output=TRUE,verbose=TRUE,width=14,height=6,band=0.6)

> SNPs <-  pig60K[pig60K$trait2 < 1e-4, 1]
> CMplot(pig60K,type="p",plot.type="m",LOG10=TRUE,highlight=SNPs,highlight.type="h",
        col=c("grey30","grey60"),highlight.col="darkgreen",highlight.cex=1.2,highlight.pch=19,
        file="jpg",dpi=300,file.output=TRUE,verbose=TRUE,width=14,height=6)

Visualize only one chromosome

> CMplot(pig60K[pig60K$Chromosome==13, ], plot.type="m",LOG10=TRUE,col=c("grey60"),highlight=SNPs,
        highlight.col="green",highlight.cex=1,highlight.pch=19,file="jpg",memo="", 
        threshold=c(1e-6,1e-4),threshold.lty=c(1,2),threshold.lwd=c(1,2), width=9,height=6,
        threshold.col=c("red","blue"),amplify=FALSE,dpi=300,file.output=TRUE,verbose=TRUE)

add genes or SNP names around the highlighted SNPs

> SNPs <- pig60K[pig60K[,5] < (0.05 / nrow(pig60K)), 1]
> genes <- paste("GENE", 1:length(SNPs), sep="_")
> set.seed(666666)
> CMplot(pig60K[,c(1:3,5)], plot.type="m",LOG10=TRUE,col=c("grey30","grey60"),highlight=SNPs,
        highlight.col=c("red","blue","green"),highlight.cex=1,highlight.pch=c(15:17), highlight.text=genes,      
        highlight.text.col=c("red","blue","green"),threshold=0.05/nrow(pig60K),threshold.lty=2,   
        amplify=FALSE,file="jpg",memo="",dpi=300,file.output=TRUE,verbose=TRUE,width=14,height=6)
#Note:
'highlight', 'highlight.text', 'highlight.text.xadj', 'highlight.text.yadj' could be vector or list, if it is a vector, 
all traits will use the same highlighted SNPs index and text, if it is a list, the length of the list should equal to the number of traits.
the order of 'highlight.text' must be consistent with 'highlight'
highlight.text.cex: value or vecter, control the size of added text
highlight.text.font: value or vecter, control the font of added text
highlight.text.xadj: value or vecter or list for multiple traits, -1, 0, 1 limited, control the position of text around the highlighted SNPs,
                     -1(left), 0(center), 1(right)
highlight.text.yadj: value or vector or list for multiple traits, same as above, -1(down), 0(center), 1(up)

Genomic Selection/Prediction(GS/GP) or other none p-values

> CMplot(cattle50K, plot.type="m", band=0.5, LOG10=FALSE, ylab="SNP effect",threshold=0.015,
        threshold.lty=2, threshold.lwd=1, threshold.col="red", amplify=TRUE, width=14,height=6,
        signal.col=NULL, chr.den.col=NULL, file="jpg",memo="",dpi=300,file.output=TRUE,
        verbose=TRUE,cex=0.8)
#Note: if signal.col=NULL, the significant SNPs will be plotted with original colors.

> cattle50K[,4:ncol(cattle50K)] <- apply(cattle50K[,4:ncol(cattle50K)], 2, 
         function(x) x*sample(c(1,-1), length(x), rep=TRUE))
> CMplot(cattle50K, type="h",plot.type="m", band=0.5, LOG10=FALSE, ylab="SNP effect",ylim=c(-0.02,0.02),
        threshold.lty=2, threshold.lwd=1, threshold.col="red", amplify=FALSE,cex=0.6,
        chr.den.col=NULL, file="jpg",memo="",dpi=300,file.output=TRUE,verbose=TRUE)

#Note: Positive and negative values are acceptable.

Multi_tracks Rectangular-Manhattan plot

SNPs <- list(
	pig60K$SNP[pig60K$trait1<1e-6],
	pig60K$SNP[pig60K$trait2<1e-6],
	pig60K$SNP[pig60K$trait3<1e-6]
)
CMplot(pig60K, plot.type="m",multracks=TRUE,threshold=c(1e-6,1e-4),threshold.lty=c(1,2), 
        threshold.lwd=c(1,1), threshold.col=c("black","grey"), amplify=TRUE,bin.size=1e6,
        chr.den.col=c("darkgreen", "yellow", "red"), signal.col=c("red","green","blue"),
        signal.cex=1, file="jpg",memo="",dpi=300,file.output=TRUE,verbose=TRUE,
        highlight=SNPs, highlight.text=SNPs, highlight.text.cex=1.4)
#Note: if you are not supposed to change the color of signal, 
          please set signal.col=NULL and highlight.col=NULL.

a. all traits in one axes:

b. all traits in separated axes:

Single_track Q-Q plot

> CMplot(pig60K,plot.type="q",box=FALSE,file="jpg",memo="",dpi=300,
    conf.int=TRUE,conf.int.col=NULL,threshold.col="red",threshold.lty=2,
    file.output=TRUE,verbose=TRUE,width=5,height=5)

Multi_tracks Q-Q plot

> pig60K$trait1[sample(1:nrow(pig60K), round(nrow(pig60K)*0.80))] <- NA
> pig60K$trait2[sample(1:nrow(pig60K), round(nrow(pig60K)*0.25))] <- NA
> CMplot(pig60K,plot.type="q",col=c("dodgerblue1", "olivedrab3", "darkgoldenrod1"),threshold=1e-6,
        ylab.pos=2,signal.pch=c(19,6,4),signal.cex=1.2,signal.col="red",conf.int=TRUE,box=FALSE,multracks=
        TRUE,cex.axis=2,file="jpg",memo="",dpi=300,file.output=TRUE,verbose=TRUE,ylim=c(0,8),width=5,height=5)

a. all traits in a axes:

b. all traits in separated axes:

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GWAS相关的曼哈顿图-SNP密度图

R包 CMplot官网链接https://github.com/YinLiLin/CMplotA high-quality drawing tool designed for Manhattan plot of genomic analysisInstallationCMplot is available on CRAN, so it can be installed with the following R code:> install.packages("CMplot")> l
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