参考内容
https://bmcbioinformatics.biomedcentral.com/articles/10.1186/s12859-019-2599-6
monocles分析差异基因利用了一个广义加和模型:generalized addictive model (depending on smoothing functions)。
Y:the gene expression level
Xi:the predictor variable that expresses the cell categorical label
fi:nonparametric function, such as cubic splines or some other smoothing functions.
Y is modeled using Tobit model
Y* is a latent variable that corresponds to predictor x
λ is the detection threshold
For identifying DE genes, chi-square likelihood ratio test