从gff3中获取fasta序列

gff3文件格式

chr1A   NRGenome    gene    1157233 1158291 .   +   .   ID=TRIAE_CS42_U_TGACv1_641506_AA2096860.1.path1;Name=TRIAE_CS42_U_TGACv1_641506_AA2096860.1
chr1A   NRGenome    mRNA    1157233 1158291 .   +   .   ID=TRIAE_CS42_U_TGACv1_641506_AA2096860.1.mrna1;Name=TRIAE_CS42_U_TGACv1_641506_AA2096860.1;Parent=TRIAE_CS42_U_TGACv1_641506_AA2096860.1.path1;coverage=100.0;identity=100.0;matches=1059;mismatches=0;indels=0;unknowns=0
chr1A   NRGenome    exon    1157233 1158291 100 +   .   ID=TRIAE_CS42_U_TGACv1_641506_AA2096860.1.mrna1.exon1;Name=TRIAE_CS42_U_TGACv1_641506_AA2096860.1;Parent=TRIAE_CS42_U_TGACv1_641506_AA2096860.1.mrna1;Target=TRIAE_CS42_U_TGACv1_641506_AA2096860.1 1 1059 +
chr1A   NRGenome    CDS 1157233 1158291 100 +   0   ID=TRIAE_CS42_U_TGACv1_641506_AA2096860.1.mrna1.cds1;Name=TRIAE_CS42_U_TGACv1_641506_AA2096860.1;Parent=TRIAE_CS42_U_TGACv1_641506_AA2096860.1.mrna1;Target=TRIAE_CS42_U_TGACv1_641506_AA2096860.1 1 1059 +
chr1A   NRGenome    gene    1159521 1162591 .   -   .   ID=TRIAE_CS42_U_TGACv1_641506_AA2096870.1.path1;Name=TRIAE_CS42_U_TGACv1_641506_AA2096870.1
chr1A   NRGenome    mRNA    1159521 1162591 .   -   .   ID=TRIAE_CS42_U_TGACv1_641506_AA2096870.1.mrna1;Name=TRIAE_CS42_U_TGACv1_641506_AA2096870.1;Parent=TRIAE_CS42_U_TGACv1_641506_AA2096870.1.path1;coverage=100.0;identity=100.0;matches=1527;mismatches=0;indels=0;unknowns=0
chr1A   NRGenome    exon    1162250 1162591 100 -   .   ID=TRIAE_CS42_U_TGACv1_641506_AA2096870.1.mrna1.exon1;Name=TRIAE_CS42_U_TGACv1_641506_AA2096870.1;Parent=TRIAE_CS42_U_TGACv1_641506_AA2096870.1.mrna1;Target=TRIAE_CS42_U_TGACv1_641506_AA2096870.1 1 342 +
chr1A   NRGenome    exon    1161953 1162150 100 -   .   ID=TRIAE_CS42_U_TGACv1_641506_AA2096870.1.mrna1.exon2;Name=TRIAE_CS42_U_TGACv1_641506_AA2096870.1;Parent=TRIAE_CS42_U_TGACv1_641506_AA2096870.1.mrna1;Target=TRIAE_CS42_U_TGACv1_641506_AA2096870.1 343 540 +
chr1A   NRGenome    exon    1161682 1161859 100 -   .   ID=TRIAE_CS42_U_TGACv1_641506_AA2096870.1.mrna1.exon3;Name=TRIAE_CS42_U_TGACv1_641506_AA2096870.1;Parent=TRIAE_CS42_U_TGACv1_641506_AA2096870.1.mrna1;Target=TRIAE_CS42_U_TGACv1_641506_AA2096870.1 541 718 +
chr1A   NRGenome    exon    1161377 1161547 100 -   .   ID=TRIAE_CS42_U_TGACv1_641506_AA2096870.1.mrna1.exon4;Name=TRIAE_CS42_U_TGACv1_641506_AA2096870.1;Parent=TRIAE_CS42_U_TGACv1_641506_AA2096870.1.mrna1;Target=TRIAE_CS42_U_TGACv1_641506_AA2096870.1 719 889 +
chr1A   NRGenome    exon    1160679 1160710 100 -   .   ID=TRIAE_CS42_U_TGACv1_641506_AA2096870.1.mrna1.exon5;Name=TRIAE_CS42_U_TGACv1_641506_AA2096870.1;Parent=TRIAE_CS42_U_TGACv1_641506_AA2096870.1.mrna1;Target=TRIAE_CS42_U_TGACv1_641506_AA2096870.1 890 921 +
chr1A   NRGenome    exon    1160535 1160577 100 -   .   ID=TRIAE_CS42_U_TGACv1_641506_AA2096870.1.mrna1.exon6;Name=TRIAE_CS42_U_TGACv1_641506_AA2096870.1;Parent=TRIAE_CS42_U_TGACv1_641506_AA2096870.1.mrna1;Target=TRIAE_CS42_U_TGACv1_641506_AA2096870.1 922 964 +
chr1A   NRGenome    exon    1160392 1160459 100 -   .   ID=TRIAE_CS42_U_TGACv1_641506_AA2096870.1.mrna1.exon7;Name=TRIAE_CS42_U_TGACv1_641506_AA2096870.1;Parent=TRIAE_CS42_U_TGACv1_641506_AA2096870.1.mrna1;Target=TRIAE_CS42_U_TGACv1_641506_AA2096870.1 965 1032 +
chr1A   NRGenome    exon    1160086 1160127 100 -   .   ID=TRIAE_CS42_U_TGACv1_641506_AA2096870.1.mrna1.exon8;Name=TRIAE_CS42_U_TGACv1_641506_AA2096870.1;Parent=TRIAE_CS42_U_TGACv1_641506_AA2096870.1.mrna1;Target=TRIAE_CS42_U_TGACv1_641506_AA2096870.1 1033 1074 +
chr1A   NRGenome    exon    1159521 1159973 100 -   .   ID=TRIAE_CS42_U_TGACv1_641506_AA2096870.1.mrna1.exon9;Name=TRIAE_CS42_U_TGACv1_641506_AA2096870.1;Parent=TRIAE_CS42_U_TGACv1_641506_AA2096870.1.mrna1;Target=TRIAE_CS42_U_TGACv1_641506_AA2096870.1 1075 1527 +
chr1A   NRGenome    CDS 1162250 1162591 100 -   0   ID=TRIAE_CS42_U_TGACv1_641506_AA2096870.1.mrna1.cds1;Name=TRIAE_CS42_U_TGACv1_641506_AA2096870.1;Parent=TRIAE_CS42_U_TGACv1_641506_AA2096870.1.mrna1;Target=TRIAE_CS42_U_TGACv1_641506_AA2096870.1 1 342 +
chr1A   NRGenome    CDS 1161953 1162150 100 -   0   ID=TRIAE_CS42_U_TGACv1_641506_AA2096870.1.mrna1.cds2;Name=TRIAE_CS42_U_TGACv1_641506_AA2096870.1;Parent=TRIAE_CS42_U_TGACv1_641506_AA2096870.1.mrna1;Target=TRIAE_CS42_U_TGACv1_641506_AA2096870.1 343 540 +
chr1A   NRGenome    CDS 1161682 1161859 100 -   0   ID=TRIAE_CS42_U_TGACv1_641506_AA2096870.1
可以使用Biopython和pandas库来解析基因组文件和gff3文件,并提取启动子序列。 首先,需要从基因组文件读取DNA序列。假设基因组文件是fasta格式,可以使用Biopython的SeqIO模块读取序列: ```python from Bio import SeqIO genome_file = "genome.fasta" genome_seq = SeqIO.read(genome_file, "fasta").seq ``` 接下来,需要从gff3文件提取基因信息和其位置。可以使用pandas库读取gff3文件,并筛选出基因信息: ```python import pandas as pd gff_file = "genome.gff3" gff_df = pd.read_csv(gff_file, sep="\t", comment="#", header=None) gff_df.columns = ["seqid", "source", "type", "start", "end", "score", "strand", "phase", "attributes"] gene_df = gff_df[gff_df["type"]=="gene"] ``` 然后,可以根据基因的位置提取其启动子序列。假设启动子长度为1000个碱基,可以根据基因的方向,从基因的上游或下游位置提取启动子序列: ```python upstream_len = 1000 promoter_seqs = [] for index, row in gene_df.iterrows(): gene_start = row["start"] gene_end = row["end"] gene_strand = row["strand"] if gene_strand == "+": promoter_start = max(gene_start - upstream_len, 0) promoter_end = gene_start else: promoter_start = gene_end promoter_end = gene_end + upstream_len if promoter_end > len(genome_seq): promoter_end = len(genome_seq) promoter_seq = genome_seq[promoter_start:promoter_end] promoter_seqs.append(promoter_seq) ``` 最后,可以将启动子序列保存到文件: ```python with open("promoters.fasta", "w") as f: for i, promoter_seq in enumerate(promoter_seqs): f.write(">promoter_{}\n{}\n".format(i+1, promoter_seq)) ```
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