Volcano plot
Application: Volcano plots is usually used as a demonstration to the changes of gene expression from two groups. Each plot in the graph represents one gene.
We take Means of qPCR from each group.
expression of gene X in B
FC= ---------------------------------------
expression of gene X in A
Using log2FC as X axis to show the change happened after treatment.
X>0, gene X up-regulated in B compared to A
X<0, gene X down-regulated in B compared to A
X near 0, there is no significant change.
Why don’t we use raw P-value data?
Because P-value is between -1 ~ 1, all the interested data will crowd in the middle.