Stringtie 自带一个脚本prepDE.py
用于计算转录组的 Raw Counts,用法如下:
Usage: prepDE.py [options]
Generates two CSV files containing the count matrices for genes and
transcripts, using the coverage values found in the output of `stringtie -e`
Options:
-h, --help show this help message and exit
-i INPUT, --input=INPUT, --in=INPUT
a folder containing all sample sub-directories, or a
text file with sample ID and path to its GTF file on
each line [default: ./]
-g G where to output the gene count matrix [default:
gene_count_matrix.csv
-t T where to output the transcript count matrix [default:
transcript_count_matrix.csv]
-l LENGTH, --length=LENGTH
the average read length [default: 75]
-p PA