如何安装footLoop软件,来分析PacBio bisulfate处理的DNA测序数据?
如何安装footLoop软件?
按照软件开发者的REASDME默认安装,用
./check_software.pl 来check安装是否成功,出现如下信息:
Please install samtools at least version 0.1.19 before proceeding!
samtools=Unknown Samtools Version!
Please install bowtie2 at least version 2.2.6 before proceeding!
bowtie2=Binary file (standard input) matches
Please make sure to install these softwares first! bedtools (v2.25.0),
bowtie2 (v2.2.6), bismark2 (v0.20.0), R (v3.4.4) … … …
看来是最主要是samtools的问题,用conda安装后,用命令samtools view测试一下,出现如下错误,
dyld: Library not loaded: @rpath/libncursesw.5.dylib Referenced
from: /Users/myang/Ananconda/anaconda3/bin/samtools Reason: image
not found zsh: abort samtools view
网上的意见主要就是,openssl升级的问题,主要在于conda的问题,于是,我就手动编译samtools的源文件samtools-0.1.19.tar.bz2,
tar -jzvf samtools-0.1.19.tar.bz2
cd samtools-0.1.19
make
此外, 要记得手动安装bismark_v0.20.0.tar.gz,
tar -zxvf bismark_v0.20.0.tar.gz
echo “export PATH=$PATH:where/to/install” >> ~/.bashrc