gatk BQSR中gtak BaseRecalibrator 出现基因组染色体号与SNP数据染色体号对应不上错误

最新推荐文章于 2024-05-14 18:00:07 发布

一只小陈啊

最新推荐文章于 2024-05-14 18:00:07 发布

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出现以下报错：

A USER ERROR has occurred: Input files reference and features have incompatible contigs: No overlapping contigs found.
reference contigs = [NC_000001.10, NT_113878.1, NT_167207.1, NC_000002.11, NC_000003.11, NC_000004.11, NT_113885.1, NT_113888.1, NC_000005.9, NC_000006.11, NC_000007.13, NT_113901.1, NC_000008.10, NT_113909.1, NT_113907.1, NC_000009.11, NT_113914.1, NT_113916.2, NT_113915.1, NT_113911.1, NC_000010.10, NC_000011.9, NT_113921.2, NC_000012.11, NC_000013.10, NC_000014.8, NC_000015.9, NC_000016.9, NC_000017.10, NT_113941.1, NT_113943.1, NT_113930.1, NT_113945.1, NC_000018.9, NT_113947.1, NC_000019.9, NT_113948.1, NT_113949.1, NC_000020.10, NC_000021.8, NT_113950.2, NC_000022.10, NC_000023.10, NC_000024.9, NT_113961.1, NT_113923.1, NT_167208.1, NT_167209.1, NT_167210.1, NT_167211.1, NT_167212.1, NT_113889.1, NT_167213.1, NT_167214.1, NT_167215.1, NT_167216.1, NT_167217.1, NT_167218.1, NT_167219.1, NT_167220.1, NT_167221.1, NT_167222.1, NT_167223.1, NT_167224.1, NT_167225.1, NT_167226.1, NT_167227.1, NT_167228.1, NT_167229.1, NT_167230.1, NT_167231.1, NT_167232.1, NT_167233.1, NT_167234.1, NT_167235.1, NT_167236.1, NT_167237.1, NT_167238.1, NT_167239.1, NT_167240.1, NT_167241.1, NT_167242.1, NT_167243.1, NW_004070864.2, NW_003571030.1, NW_003871056.3, NW_003871055.3, NW_003315905.1, NW_003315906.1, NW_003315907.1, NW_004070863.1, NW_003871057.1, NW_004070865.1, NW_003315903.1, NW_003315904.1, NW_003315908.1, NW_004504299.1, NW_003571032.1, NW_003571033.2, NW_003315909.1, NW_003571031.1, NW_003871060.1, NW_003871059.1, NW_003315910.1, NW_004775426.1, NW_003315911.1, NW_003871058.1, NW_003315912.1, NW_003315913.1, NW_004775427.1, NW_003315915.1, NW_003315916.1, NW_003571035.1, NW_003315914.1, NW_003571034.1, NW_003315920.1, NW_003571036.1, NW_003315917.2, NW_003315918.1, NW_003871061.1, NW_004775428.1, NW_003315919.1, NW_004070866.1, NW_003871063.1, NW_003315921.1, NW_004504300.1, NW_003871062.1, NW_004775429.1, NW_004166862.1, NW_003571039.1, NW_003571038.1, NW_004775430.1, NW_003871064.1, NW_003571041.1, NW_003571037.1, NW_003871065.1, NW_003315922.2, NW_003571040.1, NW_003571042.1, NW_004775431.1, NW_003871066.2, NW_003315923.1, NW_003315924.1, NW_003315928.1, NW_003871067.1, NW_003315929.1, NW_003315930.1, NW_003315931.1, NW_004504301.1, NW_004070869.1, NW_003315925.1, NW_004070867.1, NW_004070868.1, NW_003315926.1, NW_003315927.1, NW_003571043.1, NW_003871071.1, NW_003315932.1, NW_003315934.1, NW_003315935.1, NW_003871068.1, NW_004504302.1, NW_003871070.1, NW_004775432.1, NW_003871069.1, NW_003315933.1, NW_004070870.1, NW_003871075.1, NW_003871082.1, NW_003315936.1, NW_003571045.1, NW_003871073.1, NW_003871074.1, NW_003571046.1, NW_004070871.1, NW_003871081.1, NW_003871079.1, NW_003871077.1, NW_003871080.1, NW_003871078.1, NW_003871072.2, NW_003871076.1, NW_003571048.1, NW_003571049.1, NW_003871083.2, NW_003571047.1, NW_003571050.1, NW_003315938.1, NW_003315939.1, NW_003315941.1, NW_003315942.2, NW_004504303.2, NW_003315940.1, NW_003315937.1, NW_003571051.1, NW_004166863.1, NW_003315943.1, NW_003315944.1, NW_003871084.1, NW_003315945.1, NW_003871085.1, NW_003315946.1, NW_004070872.2, NW_003315952.2, NW_003315951.1, NW_003315950.2, NW_004775433.1, NW_003871090.1, NW_004166864.2, NW_003315949.1, NW_003315948.2, NW_003871091.1, NW_003871093.1, NW_003871092.1, NW_003315953.1, NW_003571052.1, NW_003871086.1, NW_003315947.1, NW_003871088.1, NW_003315954.1, NW_003315955.1, NW_003871089.1, NW_003871087.1, NW_003315956.1, NW_003315959.1, NW_003315960.1, NW_003315957.1, NW_003315958.1, NW_003315961.1, NW_003871094.1, NW_003571053.2, NW_003315962.1, NW_003315964.2, NW_003315965.1, NW_003315963.1, NW_004775434.1, NW_004166865.1, NW_003571054.1, NW_003571055.1, NW_003571056.1, NW_003571057.1, NW_003571058.1, NW_003571059.1, NW_003571060.1, NW_003571061.1, NW_003315966.1, NW_003871095.1, NW_004504304.1, NW_003571063.2, NW_003315967.1, NW_003315968.1, NW_003315969.1, NW_003315970.1, NW_004775435.1, NW_004070874.1, NW_004070873.1, NW_004070875.1, NW_003871096.1, NW_003315972.1, NW_003315971.2, NW_004504305.1, NW_004070876.1, NW_003571064.2, NW_003871098.1, NW_003871099.1, NW_004070879.1, NW_004166866.1, NW_004070880.2, NW_004070877.1, NW_004070881.1, NW_004070882.1, NW_003871100.1, NW_003871101.3, NW_004070883.1, NW_004070884.1, NW_004070885.1, NW_003871102.1, NW_004070878.1, NW_004070891.1, NW_004070892.1, NW_004070893.1, NW_004070886.1, NW_004070887.1, NW_004070888.1, NW_004070889.1, NW_004070890.2, NW_003871103.3, NT_167244.1, NT_113891.2, NT_167245.1, NT_167246.1, NT_167247.1, NT_167248.1, NT_167249.1, NT_167250.1, NT_167251.1, NC_012920.1]
  features contigs = [1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, X, Y]

1、在出现这种问题时首先，检查你的参考基因组的染色体号，例如我这里是从NCBI下载的基因组文件，发现他的染色体号是以下这样子（还有部分没展现出来）：