使用Scaffold因为缺乏完整的基因组
https://github.com/PacificBiosciences/DevNet/wiki/E.-coli-Bacterial-Assembly
该记录只使用了一部分
Instrument: PacBio RS II
Chemistry: C4
Enzyme: P6
摘要
该数据集包括一个SMRT单元格数据,该数据由PacBio RS II系统和P6-C4化学物质收集在大小为20kb的大肠杆菌K12菌株MG1655的文库中。使用SMRT Analysis 2.0或更高版本,组装此SMRT单元可产生> Q50精度的单个重叠群。
下载数据集
主要分析(仪器输出)数据
- 输入SMRT Cell数据文件(7 GB),包括可以导入到SMRT Analysis中的metadata.xml,bas.h5和bax.h5文件。
次要分析数据
- FASTQ格式的最终基因组组装(2.6 MB)。HGAP使用SMRT Portal中的默认参数运行。
E. coli Bacterial Assembly
Casey Bergman edited this page on 5 Oct 2017 · 4 revisions
Instrument: PacBio RS II
Chemistry: C4
Enzyme: P6
Summary
This dataset includes one SMRT Cell of data gathered with a PacBio RS II System and P6-C4 chemistry on a size selected 20kb library of E. coli K12 substrain MG1655. Assembling this SMRT Cell results in a single contig at >Q50 accuracy using SMRT Analysis 2.0 or higher.
Download Datasets
Primary Analysis (Instrument Output) Data
- Input SMRT Cell data files (7 GB) including metadata.xml, bas.h5, and bax.h5 files that can be imported into SMRT Analysis.
Secondary Analysis Data
- Final genome assembly in FASTQ format (2.6 MB). HGAP was run using default parameters in SMRT Portal.