- 本文是对综述《Integrated design, execution, and analysis of arrayed and pooled CRISPR genome-editing experiments》step83之后的测序数据分析进行一个概括梳理。
- CRISPResso analysis of deep-sequencing data
- Timing:10-120min
- Experimentalparameter
- Fastq file R1
- Fastq file R2
- Amplicon sequences
- sgRNAsequences
- code:
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CRISPResso \-r1 /home/user/amplicons_data/reads1.fastq.gz \-r2 /home/user/amplicons_data/reads2.fastq.gz \-a AATGTCCCC
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