celldex::HumanPrimaryCellAtlasData 报错：Cannot connect to ExperimentHub server, using ‘localHub=TRUE‘

注意seurat与seuratobject版本应该同步为2022版本

singleR 报错

进行singleR自动注释时，使用refdata=celldex::HumanPrimaryCellAtlasData() 后遇到如下报错：

Cannot connect to ExperimentHub server, using 'localHub=TRUE' instead
Using 'localHub=TRUE'
  If offline, please also see BiocManager vignette section on offline use
Error in .updateHubDB(hub_bfc, .class, url, proxy, localHub) : 
  Invalid Cache: sqlite file
  Hub has not been added to cache
  Run again with 'localHub=FALSE'
In addition: Warning message:

解决方案：

1. 在下面链接下载celldex旧版本的压缩包：
   https://bioconductor.org/packages/3.12/data/experiment/html/celldex.html

2. 将压缩包放入工作路径，使用下面代码进行本地安装：

install.packages("~/celldex_1.0.0.tar.gz", repos = NULL, type = "source")