Dimplot报错

> DimPlot(seurat_object,reduction = "tsne",group.by = "seurat_clusters")
Error: Must request at least one colour from a hue palette.

解决方法:

rownames(seurat_object@meta.data) <- names(Idents(seurat_object))

这个跑完再重新聚类

> # 去除细胞周期影响 > s.genes <- cc.genes$s.genes > g2m.genes <- cc.genes$g2m.genes > control_data1 <- CellCycleScoring( + control_data1, + s.features = s.genes, + g2m.features = g2m.genes + ) 警告: The following features are not present in the object: MCM5, PCNA, TYMS, FEN1, MCM2, MCM4, RRM1, UNG, GINS2, MCM6, CDCA7, DTL, PRIM1, UHRF1, MLF1IP, HELLS, RFC2, RPA2, NASP, RAD51AP1, GMNN, WDR76, SLBP, CCNE2, UBR7, POLD3, MSH2, ATAD2, RAD51, RRM2, CDC45, CDC6, EXO1, TIPIN, DSCC1, BLM, CASP8AP2, USP1, CLSPN, POLA1, CHAF1B, BRIP1, E2F8, not searching for symbol synonyms 警告: The following features are not present in the object: HMGB2, CDK1, NUSAP1, UBE2C, BIRC5, TPX2, TOP2A, NDC80, CKS2, NUF2, CKS1B, MKI67, TMPO, CENPF, TACC3, FAM64A, SMC4, CCNB2, CKAP2L, CKAP2, AURKB, BUB1, KIF11, ANP32E, TUBB4B, GTSE1, KIF20B, HJURP, CDCA3, HN1, CDC20, TTK, CDC25C, KIF2C, RANGAP1, NCAPD2, DLGAP5, CDCA2, CDCA8, ECT2, KIF23, HMMR, AURKA, PSRC1, ANLN, LBR, CKAP5, CENPE, CTCF, NEK2, G2E3, GAS2L3, CBX5, CENPA, not searching for symbol synonyms 错误于AddModuleScore(object = object, features = features, name = name, : The following feature lists do not have enough features present in the object: S.Score exiting...The following feature lists do not have enough features present in the object: G2M.Score exiting... 此外: 警告信息: In AddModuleScore(object = object, features = features, name = name, : Could not find enough features in the object from the following feature lists: S.Score Attempting to match case...Could not find enough features in the object from the following feature lists: G2M.Score Attempting to match case...解析报错原因
最新发布
03-20
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