- 建库流程需要特别注意,参考10x官网:
- https://www.10xgenomics.com/support/software/cell-ranger/tutorials/cr-tutorial-neutrophils
- https://kb.10xgenomics.com/hc/en-us/articles/360004024032-Can-I-process-neutrophils-or-other-granulocytes-using-10x-Single-Cell-applications-
- 中性粒细胞的寿命很短,且RNA很少,比如基因数少于200个/细胞,很容易被过滤掉。参考:
- https://zhuanlan.zhihu.com/p/668208877