质量控制判断fastqc和multiqc

对于BBduk，fastp，Trimmomatic处理之后的结果，判断一下质控结果

可使用fastqc和multiqc

安装fastqc

Babraham Bioinformatics - FastQC A Quality Control tool for High Throughput Sequence DataBabraham Bioinformatics - FastQC A Quality Control tool for High Throughput Sequence Data

 

unzip fastqc_v0.12.1.zip
vim ~/.bashrc
#将以下字符增加到~/.bashrc中
export PATH="（路径）/FastQC:$PATH"
source ~/.bashrc
fastqc --help

 

fastqc  -t 180 ~/*.fq -o out_dir

安装multiqc

conda create -n fastqc python=3.9
conda activate fastqc
conda search multiqc
conda install multiqc=1.19
multiqc --help

multiqc (dir of fastqc result)